Detailed information of TA system TA151174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 760310..761040
• Replicon: chromosome
• Accession: NZ_CP049290
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain 08-175

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: G6324_RS03610
• Protein ID: WP_023302825.1
• Coordinates: 760310..760624 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6324_RS03615
• Protein ID: WP_032620773.1
• Coordinates: 760624..761040 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6324_RS03585 (G6324_03580)	755976..757061	+	1086	Protein_694	cellulase family glycosylhydrolase	-
G6324_RS03590 (G6324_03585)	756967..758151	-	1185	WP_015570118.1	multidrug efflux MFS transporter EmrD	-
G6324_RS03595 (G6324_03590)	758327..759160	-	834	WP_032620535.1	DMT family transporter	-
G6324_RS03600 (G6324_03595)	759223..759669	-	447	WP_003861064.1	GNAT family N-acetyltransferase	-
G6324_RS03610 (G6324_03605)	760310..760624	+	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
G6324_RS03615 (G6324_03610)	760624..761040	+	417	WP_032620773.1	helix-turn-helix domain-containing protein	Antitoxin
G6324_RS03620 (G6324_03615)	761187..761285	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
G6324_RS03625 (G6324_03620)	761392..763080	+	1689	WP_023302827.1	acetolactate synthase large subunit	-
G6324_RS03630 (G6324_03625)	763084..763371	+	288	WP_015570113.1	acetolactate synthase small subunit	-
G6324_RS03635 (G6324_03630)	763497..764090	+	594	WP_003861054.1	transcriptional regulator UhpA	-
G6324_RS03640 (G6324_03635)	764087..765592	+	1506	WP_032634219.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T151174 WP_023302825.1 NZ_CP049290:760310-760624 [Enterobacter hormaechei subsp. steigerwaltii]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T151174 NZ_CP065354:c1902363-1902211 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15249.77 Da
• Isoelectric Point: 5.2068

>AT151174 WP_032620773.1 NZ_CP049290:760624-761040 [Enterobacter hormaechei subsp. steigerwaltii]
MIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANGPEIVALRKEMESVPVGI
AVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 417 bp

>AT151174 NZ_CP065354:c1902987-1902388 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACTGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATATTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATTGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCATAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: A0A837FIL2