Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4250882..4251609 Replicon chromosome
Accession NZ_CP049278
Organism Shigella boydii strain 600690

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag G5S56_RS20870 Protein ID WP_000550189.1
Coordinates 4251295..4251609 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag G5S56_RS20865 Protein ID WP_000560266.1
Coordinates 4250882..4251298 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5S56_RS20855 (4246292) 4246292..4248648 + 2357 Protein_4109 alpha-glucosidase -
G5S56_RS20860 (4248819) 4248819..4250837 + 2019 WP_078166858.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
G5S56_RS20865 (4250882) 4250882..4251298 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
G5S56_RS20870 (4251295) 4251295..4251609 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
G5S56_RS20875 (4251893) 4251893..4253029 - 1137 WP_000018685.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
G5S56_RS20880 (4253114) 4253114..4253617 + 504 WP_001295542.1 M48 family metallopeptidase -
G5S56_RS20885 (4253694) 4253694..4254386 + 693 WP_000942539.1 vancomycin high temperature exclusion protein -
G5S56_RS20890 (4254465) 4254465..4255451 + 987 WP_004994246.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T151126 WP_000550189.1 NZ_CP049278:c4251609-4251295 [Shigella boydii]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T151126 NZ_CP065341:c4800283-4799999 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT151126 WP_000560266.1 NZ_CP049278:c4251298-4250882 [Shigella boydii]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT151126 NZ_CP065341:c4800515-4800273 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References