Detailed information of TA system TA151107

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sib/-
• Location: 1101011..1101424
• Replicon: chromosome
• Accession: NZ_CP049278
• Organism: Shigella boydii strain 600690

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: G5S56_RS05215
• Protein ID: WP_223200913.1
• Coordinates: 1101011..1101133 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	sib
Locus tag	-
Coordinates	1101218..1101424 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S56_RS05190 (1096468)	1096468..1096815	+	348	WP_000631709.1	IS66 family insertion sequence element accessory protein TnpB	-
G5S56_RS05195 (1096835)	1096835..1097479	+	645	Protein_1014	IS66-like element ISSfl3 family transposase	-
G5S56_RS05205 (1098822)	1098822..1098956	+	135	Protein_1016	transposase	-
G5S56_RS05210 (1099026)	1099026..1100559	+	1534	WP_119181915.1	IS3 family transposase	-
G5S56_RS05215 (1101011)	1101011..1101133	-	123	WP_223200913.1	Hok/Gef family protein	Toxin
G5S56_RS25055 (1101078)	1101078..1101230	-	153	Protein_1019	DUF5431 family protein	-
- (1101218)	1101218..1101424	-	207	NuclAT_4	-	Antitoxin
- (1101218)	1101218..1101424	-	207	NuclAT_4	-	Antitoxin
- (1101218)	1101218..1101424	-	207	NuclAT_4	-	Antitoxin
- (1101218)	1101218..1101424	-	207	NuclAT_4	-	Antitoxin
G5S56_RS25060 (1101610)	1101610..1101708	-	99	Protein_1020	IS66 family insertion sequence element accessory protein TnpB	-
G5S56_RS05220 (1101692)	1101692..1102633	-	942	Protein_1021	IS66 family transposase	-
G5S56_RS05225 (1102653)	1102653..1102999	-	347	Protein_1022	IS66 family insertion sequence element accessory protein TnpB	-
G5S56_RS05230 (1102999)	1102999..1103376	-	378	Protein_1023	IS66-like element accessory protein TnpA	-
G5S56_RS05235 (1103443)	1103443..1104789	+	1347	WP_178966593.1	IS4-like element IS4 family transposase	-
G5S56_RS05240 (1104980)	1104980..1105738	+	759	WP_001295562.1	(2E,6E)-farnesyl-diphosphate-specific ditrans,polycis-undecaprenyl-diphosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1094246..1104789	10543
	-	inside	IScluster/Tn	-	-	1094246..1104789	10543

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4538.38 Da
• Isoelectric Point: 8.2691

>T151107 WP_223200913.1 NZ_CP049278:c1101133-1101011 [Shigella boydii]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 123 bp

>T151107 NZ_CP065253:4450898-4451266 [Streptomyces clavuligerus]
ATGCACTACCTCACCCTGCCCGAGCTGCTGAACCTCGCGGAGCGGCTGCGGGCGGCCGAGGTGCGCGACTACGGTCTGCT
GGACTCCGCCCTCGCGCGCCCCCAGGCGAGCGTCTTCGGCCAGGACGCCTACCCGGATGTCTGGCAGAAGGCCGCCGCCC
TGATGGAGTCCCTGGCCCGCAACCACGGACTGGTCGACGGCAACAAGCGGATCGCCTGGTATGCCACCTGGGTCTTTCTC
CATGTGAACGGCCATCCGCTGGACCCGGAATTCGACGTGGACGAGGCGGAACGGTTTGTTCTGGACGTCTGCCAGGGGGC
ACTCGACGTCCCCAAGATCGCGTCGGGGCTGCTGCGCTTCGCCCGCTGA

Antitoxin

Nucleotide

• Length: 207 bp

>AT151107 NZ_CP049278:c1101424-1101218 [Shigella boydii]
TTACCGTGAACTGGCTGAACGACCAGATTATTTTCAAGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATGGGCTATGATG
TGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCGTGTGGCAGAAGGACAAAAGCCCC
GTAGTTAATTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Antitoxin