Detailed information of TA system TA151037

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4207423..4208126
• Replicon: chromosome
• Accession: NZ_CP049204
• Organism: Escherichia coli strain PapM-32-1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: G6Z93_RS19935
• Protein ID: WP_164476776.1
• Coordinates: 4207423..4207764 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: G6Z93_RS19940
• Protein ID: WP_164476777.1
• Coordinates: 4207785..4208126 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z93_RS19915	4202967..4203884	+	918	WP_164476773.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
G6Z93_RS19920	4204398..4205060	-	663	WP_164476774.1	hypothetical protein	-
G6Z93_RS19925	4205064..4205774	-	711	Protein_3896	integrase arm-type DNA-binding domain-containing protein	-
G6Z93_RS19930	4206157..4207164	-	1008	WP_164476775.1	restriction endonuclease	-
G6Z93_RS19935	4207423..4207764	-	342	WP_164476776.1	TA system toxin CbtA family protein	Toxin
G6Z93_RS19940	4207785..4208126	-	342	WP_164476777.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
G6Z93_RS19945	4208137..4208679	-	543	WP_164476778.1	DNA repair protein RadC	-
G6Z93_RS19950	4208692..4209135	-	444	WP_164476779.1	antirestriction protein	-
G6Z93_RS19955	4209166..4209987	-	822	WP_164476780.1	DUF945 domain-containing protein	-
G6Z93_RS19960	4210108..4210581	-	474	WP_164476781.1	hypothetical protein	-
G6Z93_RS19965	4210653..4211105	-	453	WP_000734321.1	hypothetical protein	-
G6Z93_RS19970	4211141..4211857	-	717	WP_164476782.1	WYL domain-containing protein	-
G6Z93_RS19975	4212101..4212976	-	876	Protein_3906	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4202201..4229434	27233

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12822.70 Da
• Isoelectric Point: 5.6981

>T151037 WP_164476776.1 NZ_CP049204:c4207764-4207423 [Escherichia coli]
MKTLPATTPQAETLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYELVRIDRRGF
DWQEQSPYLRAVDILRARQATGLLKRNRISAAQ

Nucleotide

• Length: 342 bp

>T151037 NZ_CP065178:c1053938-1053684 [Pectobacterium quasiaquaticum]
ATGAACAAACGACACCAGAAAACACTGTCAGATATATTTTCCCGCCCAGTTAGCGGCTCGATAAAGTGGAACGATATTGA
ATCTTTGTTTATCGCGCTGGGTGCAGAAGTACATGAAAGGGAAGGTTCAAGGGTTGCGGTACTGCTGAAGGGTGAGAAAA
GAATTTTTCATCGTCCCCACCCTAGACCAACCACTGACAAGGGAGCGGTTAACTCCATTCGGGTATGGTTAGACAGTTTA
GGAATAAGGCCATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12895.67 Da
• Isoelectric Point: 6.3161

>AT151037 WP_164476777.1 NZ_CP049204:c4208126-4207785 [Escherichia coli]
MKSTDSENDLFLKWGLDRNVTPCFGARLVQEGNRLHYLADRASITGKFINAEYLKLDEIFPHFISQMESMLITGEMNPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT151037 NZ_CP065178:c1053687-1053346 [Pectobacterium quasiaquaticum]
ATGATTAATACACTGCAAATCGACGGCCACACGGCCGTCATCGCTTTTGACCCAGAAATTGAGATGTTTCGTGGGGAATT
TGTCGGTCTTAACGGCGGCGCGGATTTCTATGCTTACAGCGTGGAAGAGTTAAAAAAAGAAGGTGCCACATCGCTTGCCG
TATTTCTTGATGAGTGCCGTAATGATGGCATTGAACCGTATAAATCATACAGCGGTAAAGTGACTACACGCCTCACACCG
GAACGCCACGCAGCGTTAGCAGTAACGGCACAGGCTAACGGGGTGTCAATTAACGAACTGTTAAATGAAGGTGTCGATCT
CGTCATTGAACGGCATTCATAA