Detailed information of TA system TA151032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2325571..2327159
• Replicon: chromosome
• Accession: NZ_CP049204
• Organism: Escherichia coli strain PapM-32-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A085NXE9
• Locus tag: G6Z93_RS11035
• Protein ID: WP_044191965.1
• Coordinates: 2325837..2327159 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: G6Z93_RS11030
• Protein ID: WP_001613070.1
• Coordinates: 2325571..2325837 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6Z93_RS11025	2323947..2325347	+	1401	WP_164476534.1	autotransporter outer membrane beta-barrel domain-containing protein	-
G6Z93_RS11030	2325571..2325837	+	267	WP_001613070.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
G6Z93_RS11035	2325837..2327159	+	1323	WP_044191965.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
G6Z93_RS11040	2327430..2327699	+	270	Protein_2158	helix-turn-helix transcriptional regulator	-
G6Z93_RS11045	2328133..2328696	+	564	WP_001296758.1	type 1 fimbrial major subunit FimA	-
G6Z93_RS11050	2329057..2329767	+	711	WP_001195165.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	2325571..2341681	16110

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49365.00 Da
• Isoelectric Point: 8.5823

>T151032 WP_044191965.1 NZ_CP049204:2325837-2327159 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T151032 NZ_CP065178:c171604-171425 [Pectobacterium quasiaquaticum]
ATGGATAGCAGGACGTTAATAGCAGAAATCAAGGCCGATGGGTGGGAACTGATAAGAGTGAACGGAAGCCACCACCATTT
TATGCATCCTAGTAAACTGGGGTTAGTCACAATCCCCCACCCTAAGAAAGATTTACCGATAGGCACGGTAAAAAACATCA
GGAAACAAGCAGGAATATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10033.36 Da
• Isoelectric Point: 4.9632

>AT151032 WP_001613070.1 NZ_CP049204:2325571-2325837 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT151032 NZ_CP065178:c171397-170987 [Pectobacterium quasiaquaticum]
ATGTTTTATCCGATTGCAATTGAAGCAGGCGACGATACCCACGCATACGGTGTAACAGTGCCAGATCTACCGGGCTGTTT
TTCCGCAGGCGATACGCTAGACGATGCCATCGCGAATGCGAAAGAGGCGATTACCGGACATATCGAACTGCTGATCGAGA
TGGGTCAGGATATTCCTACCGTGTCTTCAGTAGGCCAATGGGCAAAAAGCGCAGAATACGCTGGGTACACGTGGGCGGTT
GTTGATATCGATGTGACGCGTTTAATGGGCGGTTCAGAAAAAATTAACGTCACGCTGCCTAAATCCCTGATTGACCGAAT
TGATCGTTGCGTGGCGAGTAATCCTGAATTTAAAAGCCGCTCAGGTTTTCTCGCGCAGGCGGCATTAGCGCGAATCTCGT
CTTCCCGATGA

Structures

Toxin

• AlphaFold DB: A0A085NXE9