Detailed information of TA system TA150853

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 47865..48134
• Replicon: plasmid p19-0821-3486
• Accession: NZ_CP049186
• Organism: Shigella sonnei strain 19.0821.3486

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: G6O60_RS24820
• Protein ID: WP_001372321.1
• Coordinates: 48009..48134 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	47865..47930 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O60_RS24785	43575..44102	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
G6O60_RS24790	44160..44393	+	234	WP_000006003.1	DUF905 family protein	-
G6O60_RS24795	44454..46477	+	2024	Protein_56	ParB/RepB/Spo0J family partition protein	-
G6O60_RS24800	46546..46980	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
G6O60_RS24805	46977..47696	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	47708..47932	+	225	NuclAT_0	-	-
-	47708..47932	+	225	NuclAT_0	-	-
-	47708..47932	+	225	NuclAT_0	-	-
-	47708..47932	+	225	NuclAT_0	-	-
G6O60_RS24810	47717..47896	-	180	WP_001309233.1	hypothetical protein	-
-	47865..47930	+	66	-	-	Antitoxin
G6O60_RS24815	47918..48067	+	150	Protein_60	plasmid maintenance protein Mok	-
G6O60_RS24820	48009..48134	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
G6O60_RS24825	48453..48749	-	297	Protein_62	hypothetical protein	-
G6O60_RS24830	49049..49345	+	297	WP_001272251.1	hypothetical protein	-
G6O60_RS24835	49456..50277	+	822	WP_001234445.1	DUF932 domain-containing protein	-
G6O60_RS24840	50574..51164	-	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
G6O60_RS24845	51499..51882	+	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
G6O60_RS24850	52076..52747	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
G6O60_RS24855	52884..53111	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T150853 WP_001372321.1 NZ_CP049186:48009-48134 [Shigella sonnei]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T150853 NZ_CP065047:5261863-5262168 [Mycobacterium kubicae]
GTGATCACACCCGGAGACATCACGCCAGGCCGCGACACCAACCAGGAACTGTACGTTGTCGTACTATCTAACACGATTCA
CCTCGCAGCCGCCACCGGCCAGGTGATCATCTGCCCCTTCATTCCCGGTGAAATCCCCAGCAGCACAATGGCTATGATCG
TCACAGTGCTGCAACCCAAAGGGGTTGTCTTACCGGAGCTCATCCAGTGGCTTCCTGTCGCGGCCCTCGACCAACCCATC
GGCAATATCGGCGGCATCGCGCTCGCTGACACGACCACGACCGCCGTCACCGCGCTCATTTCCTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT150853 NZ_CP049186:47865-47930 [Shigella sonnei]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Antitoxin