Detailed information of TA system TA150814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 274972..275809
• Replicon: chromosome
• Accession: NZ_CP049179
• Organism: Shigella sonnei strain L4094

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: G6O64_RS01315
• Protein ID: WP_000227784.1
• Coordinates: 275267..275809 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: G6O64_RS01310
• Protein ID: WP_001297137.1
• Coordinates: 274972..275283 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O64_RS01285 (269992)	269992..270939	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
G6O64_RS01290 (270961)	270961..272952	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
G6O64_RS01295 (272942)	272942..273556	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
G6O64_RS01300 (273556)	273556..273885	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
G6O64_RS01305 (273897)	273897..274787	+	891	WP_000971327.1	heme o synthase	-
G6O64_RS01310 (274972)	274972..275283	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
G6O64_RS01315 (275267)	275267..275809	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
G6O64_RS01320 (275865)	275865..276431	-	567	Protein_257	SEL1-like repeat protein	-
G6O64_RS01325 (276465)	276465..277162	+	698	WP_237158184.1	IS1-like element IS1A family transposase	-
G6O64_RS01330 (277179)	277179..277577	-	399	Protein_259	SEL1-like repeat protein	-
G6O64_RS01340 (278762)	278762..280126	+	1365	WP_001000971.1	MFS transporter	-
G6O64_RS01345 (280254)	280254..280745	-	492	WP_001138904.1	nucleotide binding protein YajQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	277946..278449	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T150814 WP_000227784.1 NZ_CP049179:275267-275809 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T150814 NZ_CP065037:c3003424-3003065 [Leifsonia sp. ZF2019]
GTGAGACGGGGAGAGGTCTACGACGTCCAGCTCGATCCCGTCGTCGAGGGGGAGGCGGCGCGGTTGCGTCCGTGCATCGT
GGTGAGCAACGAAGGGGTCAACGCCTCCGTCACCCGACGCGGTCGCGGCGTTCTCGCGTTCGTTCCGCTGACCTCGAATG
TCGCGAACGTCCACGAGTTCCAGGTGGCCGTGGACGACCCCGAGGACCTCGCCCTGATGGGGCTCGACCGGGCGTCGAAG
ATCCAGGCTGAGCAGGTGCGCGCGATCGGTCACCCGCGTATCGGCCGCTATCGCGGAGTCACGCCCGCATGGATCATGCA
CCAGGTCGACGATGCCCTCCGTTTCCATCTCTCGCTGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT150814 WP_001297137.1 NZ_CP049179:274972-275283 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT150814 NZ_CP065037:c3003642-3003421 [Leifsonia sp. ZF2019]
ATGACCAAGTTGAGTGTGAGCCTCTCGGAGGAGGACGTCGCCTACCTCGACGTCGTCGCCGCTGATTTCGCCGGCAATCG
CTCGGCGGCGATCCATCGACTGATCCGCCTCAAGCGCGAACTCGACGCCGAGGGCGCCTACGCACAGGCGTTCGACGAGT
GGGAGGCGTCCGGGGAGCAGGAGCTCTGGGACCAGACGGCGGGGGACGGTCTCGAGGAGTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3