Detailed information of TA system TA150758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2505136..2505973
• Replicon: chromosome
• Accession: NZ_CP049173
• Organism: Shigella sonnei strain 19.0820.1561

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: G6O61_RS12905
• Protein ID: WP_000227784.1
• Coordinates: 2505431..2505973 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: G6O61_RS12900
• Protein ID: WP_001297137.1
• Coordinates: 2505136..2505447 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O61_RS12875 (2500156)	2500156..2501103	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
G6O61_RS12880 (2501125)	2501125..2503116	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
G6O61_RS12885 (2503106)	2503106..2503720	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
G6O61_RS12890 (2503720)	2503720..2504049	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
G6O61_RS12895 (2504061)	2504061..2504951	+	891	WP_000971327.1	heme o synthase	-
G6O61_RS12900 (2505136)	2505136..2505447	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
G6O61_RS12905 (2505431)	2505431..2505973	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
G6O61_RS12910 (2506029)	2506029..2506595	-	567	Protein_2521	SEL1-like repeat protein	-
G6O61_RS12915 (2506629)	2506629..2507326	+	698	WP_094096600.1	IS1-like element IS1A family transposase	-
G6O61_RS12920 (2507343)	2507343..2507741	-	399	Protein_2523	SEL1-like repeat protein	-
G6O61_RS12930 (2508926)	2508926..2510290	+	1365	WP_001000971.1	MFS transporter	-
G6O61_RS12935 (2510418)	2510418..2510909	-	492	WP_001138904.1	nucleotide binding protein YajQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T150758 WP_000227784.1 NZ_CP049173:2505431-2505973 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T150758 NZ_CP065030:1706208-1706561 [Pectobacterium versatile]
GTGTGGAGAATTGAAACGACGGACGTGTTTGATAGCTGGTTCAGCGCCTTAAACGATAGGGATCGAGCTTGCGTGCTTGC
AGCACTTATCGTGTTGCGGGAAAAAGGTCCAATGTTATCTAGACCTTATGCCGATACGGTTAAAGGTTCTCGTTATAGCA
ACATGAAAGAGCTGCGTATTCAGAGCCGAGGAACGCCGATACGGGCATTTTTTGCCTTCGACCCACATCGTACAGGGATC
CTGCTCTGCGCAGGCAATAAGTCCGGCAATGAAAAGCGCTTTTACGATGAGATGCTCCCGTTTGCTGAGCGGGAGTTAAC
AAATTATCTGAAAAAATCAGAGAAGAGGGAGTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT150758 WP_001297137.1 NZ_CP049173:2505136-2505447 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT150758 NZ_CP065030:1706566-1706868 [Pectobacterium versatile]
ATGGGCAGAACGCTGGAACAGCTTCTTGCGGATGAAAAACCAGAGGTTGTGGTCGATGCTCACGCTATAGCGACAGAGAT
GCTGCTCAATATTCATCTCGCTGAACTGCGTGAGAGGGTGCAAAAAACGCAAGTAGAAATGGCACAGGTACTTGGGTTAA
AACAGCCGACCGTGGCGGTAATGGAGAAACCCGGCCGAGATCTGAAACTTTCCACGCTTAAACGCTATGTCGAAGCGACC
GGCAGTAAGCTACGTCTTGATGTTGAACTACCGGACGGCTCGCATTACGAATTTATGCTGTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3