Detailed information of TA system TA150732

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2864773..2865610
• Replicon: chromosome
• Accession: NZ_CP049171
• Organism: Shigella sonnei strain 0401930105

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: G6O59_RS14220
• Protein ID: WP_000227784.1
• Coordinates: 2864773..2865315 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: G6O59_RS14225
• Protein ID: WP_001297137.1
• Coordinates: 2865299..2865610 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O59_RS14190 (2859837)	2859837..2860328	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
G6O59_RS14195 (2860456)	2860456..2861820	-	1365	WP_001000971.1	MFS transporter	-
G6O59_RS14200 (2861939)	2861939..2862636	+	698	WP_237158184.1	IS1-like element IS1A family transposase	-
G6O59_RS14205 (2863005)	2863005..2863403	+	399	Protein_2754	SEL1-like repeat protein	-
G6O59_RS14215 (2864151)	2864151..2864717	+	567	Protein_2756	SEL1-like repeat protein	-
G6O59_RS14220 (2864773)	2864773..2865315	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
G6O59_RS14225 (2865299)	2865299..2865610	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
G6O59_RS14230 (2865795)	2865795..2866685	-	891	WP_000971327.1	heme o synthase	-
G6O59_RS14235 (2866697)	2866697..2867026	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
G6O59_RS14240 (2867026)	2867026..2867640	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
G6O59_RS14245 (2867630)	2867630..2869621	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
G6O59_RS14250 (2869643)	2869643..2870590	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T150732 WP_000227784.1 NZ_CP049171:c2865315-2864773 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T150732 NZ_CP065020:c2915738-2915340 [Sinorhizobium meliloti]
ATGCTCACCTATATGCTCGATACCAATATCTGCATCTATGTGATGAAGACCTATCCGCCGGCCGTGCGCGAAAAGTTCAA
CGGACTGGCGGAGCAGCTTTGCATATCGAGCATCACGCTGGGAGAATTACACTATGGCGCTGAAAAATCGGCCCGGCGCG
TTGAGAACCTTACGGCTATCGAACATTTCGTGGCACGGCTGGAGGTGCTGCCGTTCGCAGACAAGGCAGCGGCCCACTAC
GGGCAGGTTCGGGCTGAACTGGAGCGGACGGGAACGCCATGCGGCCCCCACGACATGCAGATCGGCGCGCATGCGCGCAG
CGAAGGGCTGATTGTCGTGACTAACAATATACGCGAGTTCGTCCGCATGCCGGGTGTAAGGGTCGAGAACTGGCTGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT150732 WP_001297137.1 NZ_CP049171:c2865610-2865299 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT150732 NZ_CP065020:c2915971-2915738 [Sinorhizobium meliloti]
TTGGCCAGTTCGACCGTATTCATCAGTAACCGTAGCCAAGCGGTGCGGCTGCCAAAGGCCGTCGCCTTTCCCGAGGGCGT
GCATCAGGTCGATATCCTCAAGATCGGCCGCAGCCGCGTGATCGTCCCCCAGGGCAAAAGATGGGACGATCTGTTCCTCA
GCGGGCCGCGCGTCAGCGAGGATTTCATGAGCGAGCGTGATCAGCCGGTGGCGGAAACACGCGAATCCTTCTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3