Detailed information of TA system TA150707

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 176209..177046
• Replicon: chromosome
• Accession: NZ_CP049169
• Organism: Shigella sonnei strain 19.1125.3493

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: G6O58_RS00840
• Protein ID: WP_000227784.1
• Coordinates: 176504..177046 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: G6O58_RS00835
• Protein ID: WP_001297137.1
• Coordinates: 176209..176520 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O58_RS00810 (171229)	171229..172176	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
G6O58_RS00815 (172198)	172198..174189	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
G6O58_RS00820 (174179)	174179..174793	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
G6O58_RS00825 (174793)	174793..175122	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
G6O58_RS00830 (175134)	175134..176024	+	891	WP_000971327.1	heme o synthase	-
G6O58_RS00835 (176209)	176209..176520	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
G6O58_RS00840 (176504)	176504..177046	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
G6O58_RS00845 (177102)	177102..177668	-	567	Protein_166	SEL1-like repeat protein	-
G6O58_RS00850 (177702)	177702..178399	+	698	WP_237158184.1	IS1-like element IS1A family transposase	-
G6O58_RS00855 (178416)	178416..178814	-	399	Protein_168	SEL1-like repeat protein	-
G6O58_RS00865 (179999)	179999..181363	+	1365	WP_001000971.1	MFS transporter	-
G6O58_RS00870 (181491)	181491..181982	-	492	WP_001138904.1	nucleotide binding protein YajQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T150707 WP_000227784.1 NZ_CP049169:176504-177046 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T150707 NZ_CP064933:c84689-84255 [Rhizobium phaseoli]
GTGAGGCTTCTGCTGGACACCAACGTCCTGTCCGAGGTGACGAAGCCGAACCCTGACATGCGCGTCTTGGCGTGGCTGGA
CAAGCTCGACGAGGATCGCACCTTCATCAGCATCGTGTCGATCGCGGAGATCCGGCGCGGTGTCGCCTTGATGGACAGCG
GACGAAAACGCGACGCGCTGGCCGAATGGCTTGCCCGGGACCTGCCGCAACGCTTCGAGCGTCGCATCATCCCGGTGGAG
GAGCCGGTTGCCTTGGCCTGGGGCGACCTGATGGGCCAGGCGAAGCGGAGCGGTCGCGGCCTTTCCTCAATGGACGGCCT
GATCGCAGCAACCGCGGTTGCTCACGATCTCACTCTGGCTATACGTAACACCAGAGATTTCGAGGGATTCGGGATCGAAC
TCGTTGATCCCTGGGTCGATCCTCTCGATCCCTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT150707 WP_001297137.1 NZ_CP049169:176209-176520 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT150707 NZ_CP064933:c84952-84686 [Rhizobium phaseoli]
ATGCAGCCATCACGGCAAGGCGATGAAAGCTGGACCGTTGCCAGCGCAAAGGCAAAGCTTTCCGAGGTTATCGAACGCGC
GCAATCCGCGCCGCAGACGATTACCCGGAACGGCAAGCCGAGCGTCGTCGTCGTCTCCGCCGAGGAATGGCAGCGCAAGA
CGGCGCGCAAGGGTACGCTTGTCGAGTTTCTGATGAGTTCGCCGCTGCGCGGCGCTGATCTGGATCTCGAGCGTCAACGC
GACGAACCGCGTGATCCTGAACTGTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3