Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4435828..4436369 Replicon chromosome
Accession NZ_CP049167
Organism Shigella sonnei strain 09163633

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag G6O57_RS22490 Protein ID WP_000615976.1
Coordinates 4436091..4436369 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag G6O57_RS22485 Protein ID WP_000729704.1
Coordinates 4435828..4436088 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6O57_RS22460 (4431297) 4431297..4433009 + 1713 Protein_4384 flagellar biosynthesis protein FlhA -
G6O57_RS22465 (4433132) 4433132..4433629 - 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
G6O57_RS22470 (4433700) 4433700..4433828 - 129 Protein_4386 ISAs1 family transposase -
G6O57_RS22475 (4433883) 4433883..4434580 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
G6O57_RS22480 (4434869) 4434869..4435642 - 774 WP_127148796.1 C40 family peptidase -
G6O57_RS22485 (4435828) 4435828..4436088 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
G6O57_RS22490 (4436091) 4436091..4436369 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
G6O57_RS22495 (4436525) 4436525..4437265 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
G6O57_RS22500 (4437236) 4437236..4438003 - 768 WP_000333380.1 class II glutamine amidotransferase -
G6O57_RS22505 (4438209) 4438209..4438787 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4434256..4447230 12974


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T150702 WP_000615976.1 NZ_CP049167:4436091-4436369 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T150702 NZ_CP064923:c5629643-5629314 [Acaryochloris marina S15]
ATGGGAGTGGTAGTCAAGCGTTTTGATGTGTTTCTAGTTAACCTCGATCCGACAGTAGAGAGTGAAATTCAGAAGACGCG
ACCGTGTATTGTGATCTCTCCTAACGAGATGAACCAATACATTGCCACAGTGATCATCGCTCCCATGACCACAAAAGGAA
GGCAATATCCAACGCGTGTTGCTTGCCAATTTCAGGGAAAAGATGGACAAATTGTTTTGGATAAAATTCGCACAGTTGAT
AAAAAACGATTAGTCAAAAAGCTAGGTCAGATCAATCAAGATGAGCAGCAAATGCTCTTATCGATCTTGACTGAAATGTT
TGCTGAATAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT150702 WP_000729704.1 NZ_CP049167:4435828-4436088 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT150702 NZ_CP064923:c5629891-5629631 [Acaryochloris marina S15]
ATGGATACAGCTATTAGGACCCGCATCGTTAAAATTGGCAACTCCCAGGGTATTCGCATTCCCAAGCTTTTATTAGAGCA
AAGTGGTCTCAATGGAGAGGTTGAGATTACCGTGCAAGGAGACTGCTTAACCATTCGTACTGCTGAATGTTCTCGTGCTG
GGTGGGATGCAGCTTTTGCTTCCATGGCCCAACATCATGACGATGTGTTTTTAGATGAAGTCAACACTACCGACTGGGAT
GATACTGAATGGGAGTGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References