Detailed information of TA system TA150682

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 59746..60172
• Replicon: plasmid p0401952027
• Accession: NZ_CP049166
• Organism: Shigella sonnei strain 0401952027

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: G6O56_RS24760
• Protein ID: WP_001372321.1
• Coordinates: 59746..59871 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	59948..60172 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6O56_RS24730 (55063)	55063..55884	-	822	WP_001234445.1	DUF932 domain-containing protein	-
G6O56_RS24735 (55995)	55995..56291	-	297	WP_001272251.1	hypothetical protein	-
G6O56_RS24740 (56591)	56591..56887	+	297	Protein_66	hypothetical protein	-
G6O56_RS24745 (56972)	56972..58585	-	1614	WP_000080200.1	IS66-like element ISEc23 family transposase	-
G6O56_RS24750 (58616)	58616..58966	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
G6O56_RS24755 (58963)	58963..59388	-	426	WP_000422741.1	transposase	-
G6O56_RS24760 (59746)	59746..59871	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
G6O56_RS24765 (59813)	59813..59962	-	150	Protein_71	plasmid maintenance protein Mok	-
G6O56_RS24770 (59984)	59984..60163	+	180	WP_001309233.1	hypothetical protein	-
- (59948)	59948..60172	-	225	NuclAT_0	-	Antitoxin
- (59948)	59948..60172	-	225	NuclAT_0	-	Antitoxin
- (59948)	59948..60172	-	225	NuclAT_0	-	Antitoxin
- (59948)	59948..60172	-	225	NuclAT_0	-	Antitoxin
G6O56_RS24775 (60184)	60184..60903	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
G6O56_RS24780 (60900)	60900..61334	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
G6O56_RS24785 (61403)	61403..63426	-	2024	Protein_75	ParB/RepB/Spo0J family partition protein	-
G6O56_RS24790 (63487)	63487..63720	-	234	WP_000006003.1	DUF905 family protein	-
G6O56_RS24795 (63778)	63778..64305	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
G6O56_RS24800 (64607)	64607..65068	+	462	Protein_78	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T150682 WP_001372321.1 NZ_CP049166:c59871-59746 [Shigella sonnei]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T150682 NZ_CP064908:c896079-895642 [Synechococcus sp. CBW1107]
ATGCGTGCCCTGCTTGACATCAACGTGATCATCGCCCTTCTGGACCGTGGCCATGTGATGCACGGGGCCGCTCGACGCTG
GATGGAGCAAGAGCTCGGCCATGGCTGGGCCACCTGCCCAATCACTGAAAACGGCGTGGTGCGGATCATGTCGCAGCCTG
CCTATCCCAATCATCGCCCTGTCGCTCAGGTGGCCGATCGCCTGGCGGAAGCGTGTAACCATTCCAGCCACGCATTCTGG
CCCGAGACGATCAGCCTGTTGGAACCCGGCCTGATCCGCTGGGAGCGACTGCTGGGCCCCCGCCAGATCACCGACAGTTA
TCTGCTGGCACTTGCCGTGACGCACGGCGGCCGATTCGTGAGCTTTGATCAGCGCATCGGCTTGGATCTTGTGCCCAAGG
CCAGTACCGAACATCTTGCGATGATCGAAAGTCCTTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT150682 NZ_CP049166:c60172-59948 [Shigella sonnei]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin