Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 2272022..2272859 Replicon chromosome
Accession NZ_CP049165
Organism Shigella sonnei strain 0401952027

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag G6O56_RS11685 Protein ID WP_000227784.1
Coordinates 2272317..2272859 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag G6O56_RS11680 Protein ID WP_001297137.1
Coordinates 2272022..2272333 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6O56_RS11655 (2267042) 2267042..2267989 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
G6O56_RS11660 (2268011) 2268011..2270002 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
G6O56_RS11665 (2269992) 2269992..2270606 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
G6O56_RS11670 (2270606) 2270606..2270935 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
G6O56_RS11675 (2270947) 2270947..2271837 + 891 WP_000971327.1 heme o synthase -
G6O56_RS11680 (2272022) 2272022..2272333 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
G6O56_RS11685 (2272317) 2272317..2272859 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
G6O56_RS11690 (2272915) 2272915..2273481 - 567 Protein_2287 SEL1-like repeat protein -
G6O56_RS11695 (2273515) 2273515..2274212 + 698 WP_237158184.1 IS1-like element IS1A family transposase -
G6O56_RS11700 (2274229) 2274229..2274627 - 399 Protein_2289 SEL1-like repeat protein -
G6O56_RS11710 (2275812) 2275812..2277176 + 1365 WP_001000971.1 MFS transporter -
G6O56_RS11715 (2277304) 2277304..2277795 - 492 WP_001138904.1 nucleotide binding protein YajQ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T150674 WP_000227784.1 NZ_CP049165:2272317-2272859 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T150674 NZ_CP064875:238591-238941 [Bacillus toyonensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGATTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGATAAAATCACTCATTTGGATGAAGTGATGATGATTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT150674 WP_001297137.1 NZ_CP049165:2272022-2272333 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT150674 NZ_CP064875:238299-238586 [Bacillus toyonensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACGGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References