Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3571068..3571609 Replicon chromosome
Accession NZ_CP049112
Organism Escherichia coli strain WF5-5-T2

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag G6R06_RS17545 Protein ID WP_000615983.1
Coordinates 3571331..3571609 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag G6R06_RS17540 Protein ID WP_000729703.1
Coordinates 3571068..3571328 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6R06_RS17520 (3566743) 3566743..3567528 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
G6R06_RS17525 (3567500) 3567500..3569212 + 1713 Protein_3424 flagellar biosynthesis protein FlhA -
G6R06_RS17530 (3569436) 3569436..3569933 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
G6R06_RS17535 (3570109) 3570109..3570858 - 750 WP_000056849.1 C40 family peptidase -
G6R06_RS17540 (3571068) 3571068..3571328 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
G6R06_RS17545 (3571331) 3571331..3571609 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
G6R06_RS17550 (3571765) 3571765..3572505 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
G6R06_RS17555 (3572476) 3572476..3573243 - 768 WP_000333380.1 class II glutamine amidotransferase -
G6R06_RS17560 (3573449) 3573449..3574027 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T150574 WP_000615983.1 NZ_CP049112:3571331-3571609 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T150574 NZ_CP064825:523563-523865 [Escherichia coli UTI89]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT150574 WP_000729703.1 NZ_CP049112:3571068-3571328 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT150574 NZ_CP064825:523901-524242 [Escherichia coli UTI89]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References