Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1804704..1805535 Replicon chromosome
Accession NZ_CP049110
Organism Escherichia coli strain WF5-5-T1

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag G5631_RS08655 Protein ID WP_000854814.1
Coordinates 1804704..1805078 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag G5631_RS08660 Protein ID WP_001285584.1
Coordinates 1805167..1805535 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5631_RS08615 (1800100) 1800100..1801266 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
G5631_RS08620 (1801385) 1801385..1801858 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
G5631_RS08625 (1802056) 1802056..1803114 + 1059 WP_001200891.1 FUSC family protein -
G5631_RS08630 (1803286) 1803286..1803615 + 330 WP_000450409.1 DUF496 family protein -
G5631_RS08635 (1803716) 1803716..1803850 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
G5631_RS08640 (1803970) 1803970..1804098 + 129 Protein_1687 transposase domain-containing protein -
G5631_RS08645 (1804387) 1804387..1804467 - 81 Protein_1688 hypothetical protein -
G5631_RS08650 (1804513) 1804513..1804707 - 195 WP_000988600.1 DUF5983 family protein -
G5631_RS08655 (1804704) 1804704..1805078 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
G5631_RS08660 (1805167) 1805167..1805535 - 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
G5631_RS08665 (1805609) 1805609..1805830 - 222 WP_000692323.1 DUF987 domain-containing protein -
G5631_RS08670 (1805893) 1805893..1806339 - 447 WP_000187523.1 RadC family protein -
G5631_RS08675 (1806336) 1806336..1807868 - 1533 WP_001350525.1 protein YeeR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T150526 WP_000854814.1 NZ_CP049110:c1805078-1804704 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T150526 NZ_CP064768:c5304509-5304126 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT150526 WP_001285584.1 NZ_CP049110:c1805535-1805167 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT150526 NZ_CP064768:c5304751-5304509 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References