Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 844693..845347 Replicon chromosome
Accession NZ_CP049110
Organism Escherichia coli strain WF5-5-T1

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag G5631_RS04135 Protein ID WP_000244777.1
Coordinates 844940..845347 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag G5631_RS04130 Protein ID WP_000354046.1
Coordinates 844693..844959 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5631_RS04105 (839862) 839862..840605 + 744 WP_000951964.1 SDR family oxidoreductase -
G5631_RS04110 (840662) 840662..842095 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
G5631_RS04115 (842140) 842140..842451 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
G5631_RS04120 (842615) 842615..843274 + 660 WP_000250274.1 hemolysin III family protein -
G5631_RS04125 (843470) 843470..844450 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
G5631_RS04130 (844693) 844693..844959 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
G5631_RS04135 (844940) 844940..845347 + 408 WP_000244777.1 protein YgfX Toxin
G5631_RS04140 (845387) 845387..845908 - 522 WP_001055874.1 flavodoxin FldB -
G5631_RS04145 (846020) 846020..846916 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
G5631_RS04150 (846941) 846941..847651 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G5631_RS04155 (847657) 847657..849390 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T150521 WP_000244777.1 NZ_CP049110:844940-845347 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T150521 NZ_CP064768:c3965924-3965607 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT150521 WP_000354046.1 NZ_CP049110:844693-844959 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT150521 NZ_CP064768:c3965614-3965327 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References