Detailed information of TA system TA150409

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 65548..65802
• Replicon: plasmid p10A_p1
• Accession: NZ_CP049082
• Organism: Escherichia coli strain p10A

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: G6P90_RS23465
• Protein ID: WP_001351576.1
• Coordinates: 65548..65754 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	65741..65802 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6P90_RS23430	61100..61501	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
G6P90_RS23435	61434..61691	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
G6P90_RS23440	61784..62437	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
G6P90_RS23445	63376..64233	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
G6P90_RS23450	64226..64708	-	483	WP_001273588.1	hypothetical protein	-
G6P90_RS23455	64701..64775	-	75	WP_001442103.1	RepA leader peptide Tap	-
G6P90_RS23460	65007..65264	-	258	WP_000083845.1	replication regulatory protein RepA	-
G6P90_RS23465	65548..65754	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	65741..65802	+	62	NuclAT_1	-	Antitoxin
-	65741..65802	+	62	NuclAT_1	-	Antitoxin
-	65741..65802	+	62	NuclAT_1	-	Antitoxin
-	65741..65802	+	62	NuclAT_1	-	Antitoxin
G6P90_RS24015	66058..66132	-	75	Protein_79	endonuclease	-
G6P90_RS23470	66378..66590	-	213	WP_001299730.1	ANR family transcriptional regulator	-
G6P90_RS23475	66726..67286	-	561	WP_000139315.1	fertility inhibition protein FinO	-
G6P90_RS23480	67389..68249	-	861	WP_000704513.1	alpha/beta hydrolase	-
G6P90_RS23485	68308..69054	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / tet(A) / aph(3')-Ia / sul3 / ant(3'')-Ia / floR / aac(3)-IIa / sitABCD	iutA / iucD / iucC / iucB / iucA	1..143163	143163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T150409 WP_001351576.1 NZ_CP049082:c65754-65548 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T150409 NZ_CP064680:4438507-4438857 [Escherichia coli K-12]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT150409 NZ_CP049082:65741-65802 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin