Detailed information of TA system TA150223

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4820971..4821806
• Replicon: chromosome
• Accession: NZ_CP048920
• Organism: Escherichia coli strain SCU-101

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q707F1
• Locus tag: G4235_RS23400
• Protein ID: WP_001094445.1
• Coordinates: 4821429..4821806 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: G4235_RS23395
• Protein ID: WP_024174331.1
• Coordinates: 4820971..4821339 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4235_RS23365	4817727..4818182	+	456	WP_000581502.1	hypothetical protein	-
G4235_RS23370	4818464..4819285	+	822	WP_021556204.1	DUF945 domain-containing protein	-
G4235_RS23375	4819285..4819530	+	246	WP_001164966.1	hypothetical protein	-
G4235_RS23380	4819624..4820097	+	474	WP_001313575.1	antirestriction protein	-
G4235_RS23385	4820113..4820589	+	477	WP_001186170.1	RadC family protein	-
G4235_RS23390	4820676..4820897	+	222	WP_000692346.1	DUF987 domain-containing protein	-
G4235_RS23395	4820971..4821339	+	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
G4235_RS23400	4821429..4821806	+	378	WP_001094445.1	TA system toxin CbtA family protein	Toxin
G4235_RS23405	4821803..4822291	+	489	WP_001534777.1	hypothetical protein	-
G4235_RS23410	4822303..4822500	+	198	WP_000838536.1	DUF957 domain-containing protein	-
G4235_RS23415	4822585..4823427	+	843	WP_001280501.1	DUF4942 domain-containing protein	-
G4235_RS23420	4824176..4825714	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14194.10 Da
• Isoelectric Point: 6.8518

>T150223 WP_001094445.1 NZ_CP048920:4821429-4821806 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T150223 NZ_CP064405:2297785-2298195 [Mycobacterium tuberculosis variant bovis BCG]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT150223 WP_024174331.1 NZ_CP048920:4820971-4821339 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT150223 NZ_CP064405:2297559-2297792 [Mycobacterium tuberculosis variant bovis BCG]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A454A7U6