Detailed information of TA system TA150219

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-ohsC/SymE(toxin)
• Location: 4564057..4564469
• Replicon: chromosome
• Accession: NZ_CP048920
• Organism: Escherichia coli strain SCU-101

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: G4235_RS22170
• Protein ID: WP_000132630.1
• Coordinates: 4564128..4564469 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	ohsC
Locus tag	-
Coordinates	4564057..4564133 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4235_RS22160	4560669..4562138	+	1470	WP_001315223.1	type I restriction-modification system subunit M	-
G4235_RS22165	4562138..4563907	+	1770	WP_000110076.1	restriction endonuclease subunit S	-
-	4564057..4564133	-	77	NuclAT_6	-	Antitoxin
-	4564057..4564133	-	77	NuclAT_6	-	Antitoxin
-	4564057..4564133	-	77	NuclAT_6	-	Antitoxin
-	4564057..4564133	-	77	NuclAT_6	-	Antitoxin
-	4564057..4564133	-	77	NuclAT_7	-	Antitoxin
-	4564057..4564133	-	77	NuclAT_7	-	Antitoxin
-	4564057..4564133	-	77	NuclAT_7	-	Antitoxin
-	4564057..4564133	-	77	NuclAT_7	-	Antitoxin
G4235_RS22170	4564128..4564469	+	342	WP_000132630.1	endoribonuclease SymE	Toxin
G4235_RS22175	4564516..4566609	-	2094	WP_000648234.1	DUF262 domain-containing protein	-
G4235_RS22180	4566707..4566787	-	81	WP_020233658.1	hypothetical protein	-
G4235_RS22185	4567015..4567935	-	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
G4235_RS22190	4568120..4569400	+	1281	WP_001304526.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T150219 WP_000132630.1 NZ_CP048920:4564128-4564469 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T150219 NZ_CP064405:c2197563-2197144 [Mycobacterium tuberculosis variant bovis BCG]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT150219 NZ_CP048920:c4564133-4564057 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin