Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4445716..4446266 Replicon chromosome
Accession NZ_CP048920
Organism Escherichia coli strain SCU-101

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag G4235_RS21610 Protein ID WP_001160966.1
Coordinates 4445716..4446030 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag G4235_RS21615 Protein ID WP_000125566.1
Coordinates 4446033..4446266 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G4235_RS21585 4441391..4442677 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
G4235_RS21590 4442677..4443666 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
G4235_RS21595 4443663..4444484 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
G4235_RS21600 4444487..4444864 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
G4235_RS21605 4444871..4445719 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
G4235_RS21610 4445716..4446030 - 315 WP_001160966.1 CcdB family protein Toxin
G4235_RS21615 4446033..4446266 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
G4235_RS21620 4446352..4446831 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
G4235_RS21625 4447023..4448885 - 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
G4235_RS21630 4448878..4449408 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
G4235_RS21635 4449516..4450847 - 1332 WP_001183207.1 MFS transporter -
G4235_RS21640 4450906..4451193 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T150216 WP_001160966.1 NZ_CP048920:c4446030-4445716 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T150216 NZ_CP064405:c2179422-2179093 [Mycobacterium tuberculosis variant bovis BCG]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT150216 WP_000125566.1 NZ_CP048920:c4446266-4446033 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT150216 NZ_CP064405:c2179796-2179419 [Mycobacterium tuberculosis variant bovis BCG]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References