Detailed information of TA system TA150215

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4243554..4244352
• Replicon: chromosome
• Accession: NZ_CP048920
• Organism: Escherichia coli strain SCU-101

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: G4235_RS20665
• Protein ID: WP_000854737.1
• Coordinates: 4243975..4244352 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P7R0L9
• Locus tag: G4235_RS20660
• Protein ID: WP_001285482.1
• Coordinates: 4243554..4243928 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4235_RS20615	4238580..4239035	-	456	WP_169711463.1	phage tail protein	-
G4235_RS20620	4239064..4239537	-	474	WP_024189672.1	phage tail protein	-
G4235_RS20625	4239639..4240211	+	573	WP_000904922.1	recombinase family protein	-
G4235_RS20630	4240432..4241253	+	822	WP_021567056.1	DUF945 domain-containing protein	-
G4235_RS20635	4241253..4241498	+	246	WP_001164966.1	hypothetical protein	-
G4235_RS20640	4241592..4242065	+	474	WP_001313575.1	antirestriction protein	-
G4235_RS20645	4242081..4242557	+	477	WP_001186170.1	RadC family protein	-
G4235_RS20650	4242620..4242841	+	222	WP_000692315.1	DUF987 domain-containing protein	-
G4235_RS20655	4242860..4243504	+	645	WP_000086759.1	hypothetical protein	-
G4235_RS20660	4243554..4243928	+	375	WP_001285482.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
G4235_RS20665	4243975..4244352	+	378	WP_000854737.1	TA system toxin CbtA family protein	Toxin
G4235_RS20670	4244349..4244841	+	493	Protein_4064	hypothetical protein	-
G4235_RS20675	4244920..4245908	-	989	Protein_4065	IS630 family transposase	-
G4235_RS20680	4246056..4246244	-	189	Protein_4066	IS66 family transposase	-
G4235_RS20685	4246305..4247438	+	1134	WP_000555410.1	IS110-like element ISEc45 family transposase	-
G4235_RS20690	4247825..4248958	+	1134	WP_000555410.1	IS110-like element ISEc45 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14240.26 Da
• Isoelectric Point: 7.8051

>T150215 WP_000854737.1 NZ_CP048920:4243975-4244352 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Nucleotide

• Length: 378 bp

>T150215 NZ_CP064405:1946276-1946524 [Mycobacterium tuberculosis variant bovis BCG]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATACGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13791.44 Da
• Isoelectric Point: 4.7511

>AT150215 WP_001285482.1 NZ_CP048920:4243554-4243928 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT150215 NZ_CP064405:1946064-1946276 [Mycobacterium tuberculosis variant bovis BCG]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0P7R0L9