Detailed information of TA system TA150199

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 885368..886202
• Replicon: chromosome
• Accession: NZ_CP048920
• Organism: Escherichia coli strain SCU-101

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0H2VDB0
• Locus tag: G4235_RS04275
• Protein ID: WP_000854688.1
• Coordinates: 885368..885745 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0H2VAY1
• Locus tag: G4235_RS04280
• Protein ID: WP_001285596.1
• Coordinates: 885822..886202 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4235_RS04245	881760..881915	-	156	WP_000729639.1	hypothetical protein	-
G4235_RS04250	882347..883281	-	935	Protein_836	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
G4235_RS04255	883274..883669	-	396	WP_000208383.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
G4235_RS04260	883738..884583	-	846	WP_001280441.1	DUF4942 domain-containing protein	-
G4235_RS04265	884668..884865	-	198	WP_000839260.1	DUF957 domain-containing protein	-
G4235_RS04270	884882..885371	-	490	Protein_840	hypothetical protein	-
G4235_RS04275	885368..885745	-	378	WP_000854688.1	TA system toxin CbtA family protein	Toxin
G4235_RS04280	885822..886202	-	381	WP_001285596.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
G4235_RS04285	886252..886896	-	645	WP_000094917.1	hypothetical protein	-
G4235_RS04290	886915..887136	-	222	WP_000692329.1	DUF987 domain-containing protein	-
G4235_RS04295	887199..887675	-	477	WP_001186726.1	RadC family protein	-
G4235_RS04300	887691..888176	-	486	WP_000849565.1	antirestriction protein	-
G4235_RS04305	888231..889052	-	822	WP_166726888.1	DUF945 domain-containing protein	-
G4235_RS25865	889133..889344	+	212	Protein_848	hypothetical protein	-
G4235_RS04315	889463..889918	-	456	WP_000581502.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	kpsM / kpsT / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF	863749..888176	24427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14014.01 Da
• Isoelectric Point: 8.5221

>T150199 WP_000854688.1 NZ_CP048920:c885745-885368 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T150199 NZ_CP064405:c755056-754673 [Mycobacterium tuberculosis variant bovis BCG]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13897.70 Da
• Isoelectric Point: 4.7959

>AT150199 WP_001285596.1 NZ_CP048920:c886202-885822 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCAVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT150199 NZ_CP064405:c755306-755151 [Mycobacterium tuberculosis variant bovis BCG]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Structures

Toxin

• AlphaFold DB: A0A0H2VDB0

Antitoxin

• AlphaFold DB: A0A0H2VAY1