Detailed information of TA system TA150024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5269654..5270279
• Replicon: chromosome
• Accession: NZ_CP048800
• Organism: Klebsiella pneumoniae strain EGKP4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G4I41_RS25890
• Protein ID: WP_238952526.1
• Coordinates: 5269654..5270037 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: G4I41_RS25895
• Protein ID: WP_004150355.1
• Coordinates: 5270037..5270279 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4I41_RS25875 (5267020)	5267020..5267922	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
G4I41_RS25880 (5267919)	5267919..5268554	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
G4I41_RS25885 (5268551)	5268551..5269480	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
G4I41_RS25890 (5269654)	5269654..5270037	-	384	WP_238952526.1	type II toxin-antitoxin system VapC family toxin	Toxin
G4I41_RS25895 (5270037)	5270037..5270279	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
G4I41_RS25900 (5270484)	5270484..5271401	+	918	WP_023302328.1	alpha/beta hydrolase	-
G4I41_RS25905 (5271415)	5271415..5272356	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
G4I41_RS25910 (5272401)	5272401..5272838	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
G4I41_RS25915 (5272835)	5272835..5273695	-	861	WP_004146232.1	virulence factor BrkB family protein	-
G4I41_RS25920 (5273689)	5273689..5274288	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14407.64 Da
• Isoelectric Point: 7.3178

>T150024 WP_238952526.1 NZ_CP048800:c5270037-5269654 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDTIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T150024 NZ_CP064376:2642160-2642378 [Shigella sonnei]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT150024 WP_004150355.1 NZ_CP048800:c5270279-5270037 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT150024 NZ_CP064376:2641760-2642134 [Shigella sonnei]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9