Detailed information of TA system TA149778

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-relB/YoeB-YefM
• Location: 930837..931339
• Replicon: chromosome
• Accession: NZ_CP048629
• Organism: Pseudomonas sp. OIL-1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: M7CQI7
• Locus tag: G3M63_RS04265
• Protein ID: WP_008938367.1
• Coordinates: 931085..931339 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: G3M63_RS04260
• Protein ID: WP_150299893.1
• Coordinates: 930837..931088 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M63_RS04225	926116..926328	-	213	WP_163981949.1	twin transmembrane helix small protein	-
G3M63_RS04230	926410..927132	+	723	WP_163984172.1	SURF1 family protein	-
G3M63_RS04235	927129..927731	+	603	WP_163981950.1	hypothetical protein	-
G3M63_RS04240	927738..928784	+	1047	WP_163981951.1	COX15/CtaA family protein	-
G3M63_RS04245	928840..929745	+	906	WP_163981952.1	heme o synthase	-
G3M63_RS04250	929736..930368	+	633	WP_163981953.1	SCO family protein	-
G3M63_RS04255	930500..930655	-	156	WP_163981954.1	hypothetical protein	-
G3M63_RS04260	930837..931088	+	252	WP_150299893.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
G3M63_RS04265	931085..931339	+	255	WP_008938367.1	Txe/YoeB family addiction module toxin	Toxin
G3M63_RS04270	931403..931852	-	450	WP_163981955.1	hypothetical protein	-
G3M63_RS04275	932242..933369	+	1128	WP_163981956.1	zinc-binding dehydrogenase	-
G3M63_RS04280	933452..934096	-	645	WP_163981957.1	DUF2959 domain-containing protein	-
G3M63_RS04285	934292..934688	-	397	Protein_847	tautomerase family protein	-
G3M63_RS04290	934797..935195	-	399	Protein_848	fosfomycin resistance glutathione transferase	-
G3M63_RS04295	935302..935937	-	636	WP_163981958.1	DUF2971 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	fosF	-	930837..940089	9252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10236.82 Da
• Isoelectric Point: 9.8162

>T149778 WP_008938367.1 NZ_CP048629:931085-931339 [Pseudomonas sp. OIL-1]
MKLIFSENAWEDYLYWQKTDKKILNRINKLIKETKREPFEGVGKPEPLKHSLAGYWSRRINEEHRIVYKVTDDALLIAQL
RYHY

Nucleotide

• Length: 255 bp

>T149778 NZ_CP064241:c4976981-4976064 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9255.47 Da
• Isoelectric Point: 4.4621

>AT149778 WP_150299893.1 NZ_CP048629:930837-931088 [Pseudomonas sp. OIL-1]
MDAISYTAARTNLAKTMEQVCEDHSPVIITRSKSQSVVMISLDDYEALQETAYLLRAPKNARRLLESVAELEQGGGQEKE
LLE

Nucleotide

• Length: 252 bp

>AT149778 NZ_CP064241:c4977745-4976987 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• AlphaFold DB: M7CQI7