Detailed information of TA system TA149772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 2677257..2678500
• Replicon: chromosome
• Accession: NZ_CP048618
• Organism: Legionella pneumophila strain ERS1305867

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: GCO85_RS11950
• Protein ID: WP_061636180.1
• Coordinates: 2677562..2678500 (+)
• Length: 313 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: -
• Locus tag: GCO85_RS11945
• Protein ID: WP_027224429.1
• Coordinates: 2677257..2677565 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCO85_RS11920	2672931..2673128	-	198	WP_014842401.1	hypothetical protein	-
GCO85_RS11925	2673148..2674374	-	1227	WP_015961555.1	MFS transporter	-
GCO85_RS11930	2675010..2675483	+	474	WP_027224431.1	hypothetical protein	-
GCO85_RS11935	2675673..2676590	+	918	WP_061636182.1	LysR family transcriptional regulator	-
GCO85_RS11940	2677033..2677260	+	228	WP_061636181.1	helix-turn-helix transcriptional regulator	-
GCO85_RS11945	2677257..2677565	+	309	WP_027224429.1	HipA N-terminal domain-containing protein	Antitoxin
GCO85_RS11950	2677562..2678500	+	939	WP_061636180.1	HipA domain-containing protein	Toxin
GCO85_RS11955	2678663..2679373	-	711	WP_027225948.1	outer membrane beta-barrel protein	-
GCO85_RS11960	2679855..2680148	-	294	WP_027225949.1	SNARE domain-containing protein	-
GCO85_RS11965	2680176..2681078	-	903	WP_061636179.1	hypothetical protein	-
GCO85_RS11970	2681241..2682695	+	1455	WP_061636178.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (53-279)

Antitoxin

• HipA, N-terminal subdomain 1 (5-98)

Sequences

Toxin

Protein

• Length: 313 a.a.
• Molecular weight: 36078.66 Da
• Isoelectric Point: 9.3347

>T149772 WP_061636180.1 NZ_CP048618:2677562-2678500 [Legionella pneumophila]
MKRCPITYEKISDQENYSQRGLRLLSPQLKNLSPLDLSADEQRQEAIARVGKTSIQGVQKKLSAKLKIKEGCFEIVDQNS
HYILKPQSDIYPELPENEAITMTLAKTIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
SMEKVIAVIEQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITRDRKISISPAYDLLNSTIAQKNTKEEIALPLKG
KKNNLTKSDFFNYFAVEKLGLNQNVINGIAQEFHQAIPEWRELISFSFLSQPMQEKYLQLLDQRCKRLNFFD

Nucleotide

• Length: 939 bp

>T149772 NZ_CP064241:879883-880290 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11586.38 Da
• Isoelectric Point: 4.5859

>AT149772 WP_027224429.1 NZ_CP048618:2677257-2677565 [Legionella pneumophila]
MRKAYVSVNGIKAGILEELQSGKYQFSYFDDYHGAPVSLTMPLTNKVYDFDVFPPFFEGLLPEGIMLEALLRKYKIDKND
YFGQLIIVGQDVVGAVTIEEIR

Nucleotide

• Length: 309 bp

>AT149772 NZ_CP064241:879636-879902 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA