Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2388421..2388983 Replicon chromosome
Accession NZ_CP048603
Organism Escherichia coli strain ChlosBP-23-1

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag G3M71_RS11375 Protein ID WP_000605675.1
Coordinates 2388421..2388699 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag G3M71_RS11380 Protein ID WP_000781370.1
Coordinates 2388699..2388983 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M71_RS11350 2384008..2384439 - 432 WP_000152305.1 peroxiredoxin OsmC -
G3M71_RS11355 2384784..2384999 + 216 WP_000495766.1 biofilm-dependent modulation protein -
G3M71_RS11360 2385101..2385238 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
G3M71_RS11365 2385394..2387091 + 1698 WP_000433462.1 malate dehydrogenase -
G3M71_RS11370 2387225..2388235 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
G3M71_RS11375 2388421..2388699 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G3M71_RS11380 2388699..2388983 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
G3M71_RS11385 2389212..2389865 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
G3M71_RS11390 2389858..2390742 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
G3M71_RS11395 2390755..2393802 - 3048 WP_015912497.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T149670 WP_000605675.1 NZ_CP048603:2388421-2388699 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T149670 NZ_CP064212:c132690-132220 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT149670 WP_000781370.1 NZ_CP048603:2388699-2388983 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT149670 NZ_CP064212:c133130-132687 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References