Detailed information of TA system TA149479

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 3960241..3960653
• Replicon: chromosome
• Accession: NZ_CP048439
• Organism: Escherichia coli strain NBRC 3301

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: GXM24_RS19295
• Protein ID: WP_000132601.1
• Coordinates: 3960312..3960653 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	3960241..3960317 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GXM24_RS19285	3957104..3958693	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
GXM24_RS19290	3958690..3960084	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
-	3960241..3960317	-	77	NuclAT_14	-	Antitoxin
-	3960241..3960317	-	77	NuclAT_14	-	Antitoxin
-	3960241..3960317	-	77	NuclAT_14	-	Antitoxin
-	3960241..3960317	-	77	NuclAT_14	-	Antitoxin
-	3960241..3960317	-	77	NuclAT_15	-	Antitoxin
-	3960241..3960317	-	77	NuclAT_15	-	Antitoxin
-	3960241..3960317	-	77	NuclAT_15	-	Antitoxin
-	3960241..3960317	-	77	NuclAT_15	-	Antitoxin
GXM24_RS19295	3960312..3960653	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
GXM24_RS19300	3960815..3962194	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
GXM24_RS19305	3962194..3963240	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T149479 WP_000132601.1 NZ_CP048439:3960312-3960653 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T149479 NZ_CP064116:66493-66903 [Enterobacter hormaechei]
GTGAACAAAATGTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGGGGTCACCGCATTGTGGTTTCGGCCATCACCTGGGCCGAAGTGTCACTGGCATCACAGGAAG
CAGGACCAGCCGCACAGAAGCTGGCTGACGCCTTCGCTGCCCGCCTTGATGCCATCCTGCCCTGGGACCGTACCGCGGTG
GACGCGACCACGGAAATTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGGCCGAACGACACGGCGATCGCCGGGCA
CGCCATCGCCGCCGGTGCCGTGCTGGTGACCAATAATGTGCGGGAATTTGAGCGGGTGCCGGGTCTGGTGCTGGAAGACT
GGGTAAGATAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT149479 NZ_CP048439:c3960317-3960241 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin