Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2853752..2854350 Replicon chromosome
Accession NZ_CP048436
Organism Flavonifractor plautii strain JCM 32125

Toxin (Protein)


Gene name mazF Uniprot ID G9YW30
Locus tag GXM20_RS14100 Protein ID WP_007494762.1
Coordinates 2854012..2854350 (+) Length 113 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G9YW29
Locus tag GXM20_RS14095 Protein ID WP_007494759.1
Coordinates 2853752..2854018 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GXM20_RS14080 2850296..2851486 - 1191 WP_163390212.1 IS256 family transposase -
GXM20_RS14085 2851620..2852810 - 1191 WP_024725322.1 IS256 family transposase -
GXM20_RS14090 2852853..2853674 - 822 WP_163390357.1 DUF3991 and TOPRIM domain-containing protein -
GXM20_RS14095 2853752..2854018 + 267 WP_007494759.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GXM20_RS14100 2854012..2854350 + 339 WP_007494762.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GXM20_RS14105 2854397..2855857 - 1461 Protein_2782 hypothetical protein -
GXM20_RS14110 2855854..2856276 - 423 WP_007494771.1 hypothetical protein -
GXM20_RS14115 2856599..2857414 - 816 WP_163390359.1 hypothetical protein -
GXM20_RS14120 2857411..2858255 - 845 Protein_2785 DUF4314 domain-containing protein -
GXM20_RS14125 2858449..2859159 - 711 WP_163390361.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-96)

Antitoxin

Antitoxin PemI-like

(1-88)


Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 12722.43 Da        Isoelectric Point: 5.5275

>T149450 WP_007494762.1 NZ_CP048436:2854012-2854350 [Flavonifractor plautii]
MVTIFEQGDIVYLDFDPQSGHEQKGRRPALVISNNLFNRVSSLTMVCPITHTDRGHPFHLRLDERTRTDGVILCDQARML
DLNSRHASFVERAPEDISSEAVDMVIGFLEPK

Download         Length: 339 bp

>T149450 NZ_CP064103:4512540-4512797 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10048.34 Da        Isoelectric Point: 4.8668

>AT149450 WP_007494759.1 NZ_CP048436:2853752-2854018 [Flavonifractor plautii]
MYATIQKWGNSQGLRIPKALLDAVGLKEHDRVELVQMDDGINIRRASSIPHRTLEERLTAFYGKPIDQIDRIEAEELDWG
KAEGSEAW

Download         Length: 267 bp

>AT149450 NZ_CP064103:4512809-4513354 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGATCCTGATGAAACCGGAGCACTGACGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A174IQD2

Antitoxin

Source ID Structure
AlphaFold DB A0A174ME76

References