Detailed information of TA system TA149294

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2785541..2786385
• Replicon: chromosome
• Accession: NZ_CP048376
• Organism: Escherichia coli strain 38

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: GZS06_RS13405
• Protein ID: WP_000854686.1
• Coordinates: 2785541..2785924 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: GZS06_RS13410
• Protein ID: WP_001285602.1
• Coordinates: 2786005..2786385 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS06_RS13365	2780544..2781035	-	492	WP_001297187.1	DUF1097 domain-containing protein	-
GZS06_RS13370	2781137..2781691	-	555	WP_001001902.1	molecular chaperone YcdY	-
GZS06_RS13375	2781715..2782452	-	738	WP_000283664.1	zinc-binding phosphatase	-
GZS06_RS13380	2782507..2783445	-	939	WP_000351311.1	glyoxylate/hydroxypyruvate reductase GhrA	-
GZS06_RS13390	2783916..2784757	-	842	Protein_2623	DUF4942 domain-containing protein	-
GZS06_RS13395	2784842..2785039	-	198	WP_000839253.1	DUF957 domain-containing protein	-
GZS06_RS13400	2785056..2785544	-	489	WP_032180032.1	hypothetical protein	-
GZS06_RS13405	2785541..2785924	-	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
GZS06_RS13410	2786005..2786385	-	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GZS06_RS13415	2786396..2787079	-	684	WP_000086768.1	hypothetical protein	-
GZS06_RS13420	2787098..2787319	-	222	WP_000692298.1	DUF987 domain-containing protein	-
GZS06_RS13425	2787382..2787858	-	477	WP_001186726.1	RadC family protein	-
GZS06_RS13430	2787874..2788359	-	486	WP_000214307.1	antirestriction protein	-
GZS06_RS13435	2788451..2789272	-	822	WP_001761104.1	DUF945 domain-containing protein	-
GZS06_RS13440	2789373..2789581	-	209	Protein_2633	DUF905 family protein	-
GZS06_RS13445	2789682..2790137	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T149294 WP_000854686.1 NZ_CP048376:c2785924-2785541 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T149294 NZ_CP063992:1406795-1407079 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT149294 WP_001285602.1 NZ_CP048376:c2786385-2786005 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT149294 NZ_CP063992:1406563-1406805 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA