Detailed information of TA system TA149278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 630893..631692
• Replicon: chromosome
• Accession: NZ_CP048376
• Organism: Escherichia coli strain 38

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: S1NYM6
• Locus tag: GZS06_RS03040
• Protein ID: WP_000347251.1
• Coordinates: 630893..631357 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: GZS06_RS03045
• Protein ID: WP_001307405.1
• Coordinates: 631357..631692 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS06_RS03010	625894..626328	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
GZS06_RS03015	626346..627224	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
GZS06_RS03020	627214..627993	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
GZS06_RS03025	628004..628477	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
GZS06_RS03030	628500..629780	-	1281	WP_000681903.1	tagatose-bisphosphate aldolase subunit KbaZ	-
GZS06_RS03035	630029..630838	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
GZS06_RS03040	630893..631357	-	465	WP_000347251.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
GZS06_RS03045	631357..631692	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
GZS06_RS03050	631841..633412	-	1572	WP_001273761.1	galactarate dehydratase	-
GZS06_RS03055	633787..635121	+	1335	WP_000599652.1	galactarate/glucarate/glycerate transporter GarP	-
GZS06_RS03060	635137..635907	+	771	WP_001058214.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17777.14 Da
• Isoelectric Point: 9.4947

>T149278 WP_000347251.1 NZ_CP048376:c631357-630893 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T149278 NZ_CP063983:c3093174-3092797 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT149278 WP_001307405.1 NZ_CP048376:c631692-631357 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT149278 NZ_CP063983:c3093631-3093263 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829CJ20

Antitoxin

• AlphaFold DB: A0A0E0XVC7