Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4207142..4207737 Replicon chromosome
Accession NZ_CP048371
Organism Escherichia coli strain 163

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag GZS08_RS20030 Protein ID WP_000239579.1
Coordinates 4207142..4207492 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag GZS08_RS20035 Protein ID WP_001223208.1
Coordinates 4207486..4207737 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS08_RS20010 4202589..4203613 - 1025 Protein_3915 ABC transporter permease -
GZS08_RS20015 4203624..4205126 - 1503 WP_000205784.1 sugar ABC transporter ATP-binding protein -
GZS08_RS20020 4205266..4206222 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
GZS08_RS20025 4206532..4207062 + 531 WP_000055072.1 inorganic diphosphatase -
GZS08_RS20030 4207142..4207492 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
GZS08_RS20035 4207486..4207737 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
GZS08_RS20040 4207949..4208290 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
GZS08_RS20045 4208293..4212072 - 3780 WP_000060978.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T149271 WP_000239579.1 NZ_CP048371:c4207492-4207142 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T149271 NZ_CP063983:1919610-1919864 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT149271 WP_001223208.1 NZ_CP048371:c4207737-4207486 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT149271 NZ_CP063983:1919362-1919613 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References