Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1857933..1858435 Replicon chromosome
Accession NZ_CP048371
Organism Escherichia coli strain 163

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GZS08_RS08740 Protein ID WP_000767829.1
Coordinates 1858181..1858435 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GZS08_RS08735 Protein ID WP_001259255.1
Coordinates 1857933..1858184 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS08_RS08710 1853110..1854177 - 1068 WP_046464156.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
GZS08_RS08715 1854177..1855247 - 1071 WP_000108945.1 histidinol-phosphate transaminase -
GZS08_RS08720 1855244..1856548 - 1305 WP_001446927.1 histidinol dehydrogenase -
GZS08_RS08725 1856554..1857453 - 900 WP_032329340.1 ATP phosphoribosyltransferase -
GZS08_RS08730 1857599..1857649 - 51 WP_001364200.1 his operon leader peptide -
GZS08_RS08735 1857933..1858184 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GZS08_RS08740 1858181..1858435 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GZS08_RS08745 1858518..1859342 + 825 WP_000754749.1 SDR family oxidoreductase -
GZS08_RS08750 1859388..1860317 + 930 WP_000803351.1 LysR family transcriptional regulator -
GZS08_RS08755 1860532..1860594 + 63 WP_010723108.1 membrane protein YoeI -
GZS08_RS08760 1860584..1861942 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GZS08_RS08765 1862121..1863179 + 1059 WP_000492321.1 transport protein YeeE -
GZS08_RS08770 1863193..1863420 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T149254 WP_000767829.1 NZ_CP048371:1858181-1858435 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T149254 NZ_CP063980:181153-181248 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT149254 WP_001259255.1 NZ_CP048371:1857933-1858184 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT149254 NZ_CP063980:c181347-181283 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATTGTGGTAGGGTGTCTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References