Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4408287..4409122 Replicon chromosome
Accession NZ_CP048330
Organism Escherichia coli strain 10

Toxin (Protein)


Gene name yeeV Uniprot ID A0A3P5DZK2
Locus tag GZS13_RS21530 Protein ID WP_060578072.1
Coordinates 4408745..4409122 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A3P5E0D5
Locus tag GZS13_RS21525 Protein ID WP_060578073.1
Coordinates 4408287..4408655 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS13_RS21490 4404082..4404753 + 672 WP_060578077.1 hypothetical protein -
GZS13_RS21495 4404766..4405176 + 411 WP_060578076.1 hypothetical protein -
GZS13_RS21500 4405397..4406215 + 819 WP_060578075.1 DUF945 domain-containing protein -
GZS13_RS21505 4406307..4406792 + 486 WP_000214415.1 antirestriction protein -
GZS13_RS21510 4406808..4407284 + 477 WP_069067380.1 RadC family protein -
GZS13_RS21515 4407353..4407574 + 222 WP_000692323.1 DUF987 domain-containing protein -
GZS13_RS21520 4407593..4408276 + 684 WP_125073544.1 antitoxin of toxin-antitoxin stability system -
GZS13_RS21525 4408287..4408655 + 369 WP_060578073.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
GZS13_RS21530 4408745..4409122 + 378 WP_060578072.1 TA system toxin CbtA family protein Toxin
GZS13_RS21535 4409119..4409607 + 489 WP_000761718.1 hypothetical protein -
GZS13_RS21540 4409619..4409816 + 198 WP_000839256.1 DUF957 domain-containing protein -
GZS13_RS21545 4409901..4410743 + 843 WP_060578071.1 DUF4942 domain-containing protein -
GZS13_RS21550 4411492..4413030 + 1539 WP_001187173.1 lysine decarboxylation/transport transcriptional activator CadC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14208.17 Da        Isoelectric Point: 7.2920

>T149092 WP_060578072.1 NZ_CP048330:4408745-4409122 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Download         Length: 378 bp

>T149092 NZ_CP063927:2008564-2008782 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13810.52 Da        Isoelectric Point: 6.3177

>AT149092 WP_060578073.1 NZ_CP048330:4408287-4408655 [Escherichia coli]
VSDSRHETNYPDDHNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT149092 NZ_CP063927:2008163-2008537 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P5DZK2

Antitoxin

Source ID Structure
AlphaFold DB A0A3P5E0D5

References