Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3758783..3759324 Replicon chromosome
Accession NZ_CP048326
Organism Escherichia coli strain 61

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag GZS14_RS18135 Protein ID WP_000615976.1
Coordinates 3759046..3759324 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag GZS14_RS18130 Protein ID WP_000729704.1
Coordinates 3758783..3759043 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS14_RS18105 3754566..3755351 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
GZS14_RS18110 3755323..3757035 + 1713 Protein_3536 flagellar biosynthesis protein FlhA -
GZS14_RS18115 3757140..3757418 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
GZS14_RS18120 3757411..3757767 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
GZS14_RS18125 3757824..3758597 - 774 WP_000543899.1 C40 family peptidase -
GZS14_RS18130 3758783..3759043 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GZS14_RS18135 3759046..3759324 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GZS14_RS18140 3759480..3760220 + 741 WP_001225679.1 murein L,D-transpeptidase -
GZS14_RS18145 3760191..3760958 - 768 WP_000333380.1 class II glutamine amidotransferase -
GZS14_RS18150 3761164..3761742 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 3751606..3785309 33703


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T149067 WP_000615976.1 NZ_CP048326:3759046-3759324 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T149067 NZ_CP063922:4439552-4439869 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAAATGCGCAATGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT149067 WP_000729704.1 NZ_CP048326:3758783-3759043 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT149067 NZ_CP063922:4439862-4440149 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References