Detailed information of TA system TA148734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 102830..103455
• Replicon: plasmid pTEM1-GZEC065
• Accession: NZ_CP048026
• Organism: Escherichia coli strain GZEC065

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GWG09_RS23480
• Protein ID: WP_000911313.1
• Coordinates: 103057..103455 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: GWG09_RS23475
• Protein ID: WP_000450520.1
• Coordinates: 102830..103057 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWG09_RS23475	102830..103057	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
GWG09_RS23480	103057..103455	+	399	WP_000911313.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GWG09_RS23485	103464..105617	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
GWG09_RS23490	105870..106601	-	732	WP_023565183.1	complement resistance protein TraT	-
GWG09_RS23495	106633..107130	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / mph(A) / aph(3')-Ia / aadA2 / dfrA12 / oqxB / oqxA / tet(A) / blaTEM-1B / sitABCD	iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..142236	142236

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T148734 WP_000911313.1 NZ_CP048026:103057-103455 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T148734 NZ_CP063860:c68776-68618 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGCTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAAATTCGTTACAGAGACGGGGACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT148734 WP_000450520.1 NZ_CP048026:102830-103057 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT148734 NZ_CP063860:c68976-68828 [Klebsiella pneumoniae]
CAGGGTGCAGACATATGGGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGT
GTTGTGTGGCAGAAAGAAGATAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCATCCCATATGTCT

Structures

Antitoxin

• AlphaFold DB: V0VCB2