Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4119860..4120401 Replicon chromosome
Accession NZ_CP048025
Organism Escherichia coli strain GZEC065

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag GWG09_RS19630 Protein ID WP_000615982.1
Coordinates 4119860..4120138 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag GWG09_RS19635 Protein ID WP_000729704.1
Coordinates 4120141..4120401 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWG09_RS19615 4117442..4118020 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
GWG09_RS19620 4118226..4118993 + 768 WP_000333380.1 class II glutamine amidotransferase -
GWG09_RS19625 4118964..4119704 - 741 WP_001225679.1 murein L,D-transpeptidase -
GWG09_RS19630 4119860..4120138 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GWG09_RS19635 4120141..4120401 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GWG09_RS19640 4120587..4121360 + 774 WP_000543899.1 C40 family peptidase -
GWG09_RS19645 4121417..4121773 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
GWG09_RS19650 4121766..4122044 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
GWG09_RS19655 4122149..4123861 - 1713 Protein_3827 flagellar biosynthesis protein FlhA -
GWG09_RS19660 4123833..4124618 + 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T148727 WP_000615982.1 NZ_CP048025:c4120138-4119860 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T148727 NZ_CP063858:2879181-2879564 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT148727 WP_000729704.1 NZ_CP048025:c4120401-4120141 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT148727 NZ_CP063858:2878939-2879181 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References