Detailed information of TA system TA148720

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2320604..2321445
• Replicon: chromosome
• Accession: NZ_CP048025
• Organism: Escherichia coli strain GZEC065

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1M0WJM1
• Locus tag: GWG09_RS11175
• Protein ID: WP_000854822.1
• Coordinates: 2320604..2320987 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: GWG09_RS11180
• Protein ID: WP_001285610.1
• Coordinates: 2321077..2321445 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWG09_RS11145	2316250..2318091	+	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-
GWG09_RS11150	2318169..2318675	-	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
GWG09_RS11160	2318974..2319819	-	846	WP_001280435.1	DUF4942 domain-containing protein	-
GWG09_RS11165	2319916..2320113	-	198	WP_000839263.1	DUF957 domain-containing protein	-
GWG09_RS11170	2320125..2320613	-	489	WP_001177592.1	hypothetical protein	-
GWG09_RS11175	2320604..2320987	-	384	WP_000854822.1	type IV toxin-antitoxin system toxin CbtA	Toxin
GWG09_RS11180	2321077..2321445	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GWG09_RS11185	2321525..2321746	-	222	WP_000692350.1	DUF987 domain-containing protein	-
GWG09_RS11190	2321815..2322291	-	477	WP_072661507.1	RadC family protein	-
GWG09_RS11195	2322307..2322792	-	486	WP_000214315.1	antirestriction protein	-
GWG09_RS11200	2322883..2323701	-	819	WP_001234702.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14295.32 Da
• Isoelectric Point: 8.2830

>T148720 WP_000854822.1 NZ_CP048025:c2320987-2320604 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHQESKRCN

Nucleotide

• Length: 384 bp

>T148720 NZ_CP063856:4570119-4570604 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTAATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTAGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT148720 WP_001285610.1 NZ_CP048025:c2321445-2321077 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT148720 NZ_CP063856:4569829-4570122 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A1M0WJM1