Detailed information of TA system TA148589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4687165..4688738
• Replicon: chromosome
• Accession: NZ_CP047962
• Organism: Aeromonas media strain MC64

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GWI30_RS21615
• Protein ID: WP_161507801.1
• Coordinates: 4687165..4688484 (-)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GWI30_RS21620
• Protein ID: WP_128296259.1
• Coordinates: 4688484..4688738 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWI30_RS21600	4682565..4683482	+	918	WP_161507887.1	ABC transporter substrate-binding protein	-
GWI30_RS21605	4683479..4685458	+	1980	WP_161507800.1	Fe(3+)-hydroxamate ABC transporter permease FhuB	-
GWI30_RS21610	4685512..4686849	-	1338	WP_004575061.1	IS4-like element ISAeme15 family transposase	-
GWI30_RS21615	4687165..4688484	-	1320	WP_161507801.1	type II toxin-antitoxin system HipA family toxin	Toxin
GWI30_RS21620	4688484..4688738	-	255	WP_128296259.1	helix-turn-helix domain-containing protein	Antitoxin
GWI30_RS21625	4688910..4689338	-	429	WP_128296257.1	DUF86 domain-containing protein	-
GWI30_RS21630	4689955..4690578	+	624	WP_161507802.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4687165..4702342	15177
	-	flank	IS/Tn	-	-	4685512..4686849	1337

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49477.59 Da
• Isoelectric Point: 6.8283

>T148589 WP_161507801.1 NZ_CP047962:c4688484-4687165 [Aeromonas media]
MANLAVYMNGYRVGTFAKATSGAHHFQYHESWLAQPGSRPISLSMPLRHQAYRGDEAYNFFDNLLPDNPEVRNRVVARYQ
AVSTQPFDLLSCIGQDSVGALQLVTEGYDVPDVRRIDYKALSDDELEQILTSYKSGIPLGMVREQEEFRISIAGAQEKTA
LLYLDGRWYLPLNATPTTHIIKLPIGKIESHSYSIDLSQSVENEYLCTLIAKEFGLPVPHCFMMQIGQVKALAVERFDRR
YAADRSWIMRLPQEDFCQVLNVPSARKYESHGGPGITDIMTILLGSTTPEQDRYLFMKSQVLFWLLAATDGHAKNFSVFI
EPEGRFRLTPFYDILSVYPVFGGRGLNRRDAKLAMGLASSKGKKYAIEQIFPRHFFQTAKAVGFERSAMEEILTELADAV
DAVIERVTQQLPASFPSTISNAILEGLKARSARLTMGWY

Nucleotide

• Length: 1320 bp

>T148589 NZ_CP063804:364928-365353 [Mycobacterium orygis]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9302.63 Da
• Isoelectric Point: 7.9850

>AT148589 WP_128296259.1 NZ_CP047962:c4688738-4688484 [Aeromonas media]
MKVTSAKQLSACIKDARLSQKLSQGKVAEKVGIRQDTVSSFELNPESTKLETLFKILAALELSLDIQPRNQSPDSPASGW
KEEW

Nucleotide

• Length: 255 bp

>AT148589 NZ_CP063804:364710-364931 [Mycobacterium orygis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA