Detailed information of TA system TA148588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2020944..2022508
• Replicon: chromosome
• Accession: NZ_CP047962
• Organism: Aeromonas media strain MC64

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A2S4TP05
• Locus tag: GWI30_RS09530
• Protein ID: WP_005329232.1
• Coordinates: 2020944..2022254 (-)
• Length: 437 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GWI30_RS09535
• Protein ID: WP_005329233.1
• Coordinates: 2022254..2022508 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWI30_RS09515	2016382..2018940	+	2559	WP_161507841.1	DEAD/DEAH box helicase family protein	-
GWI30_RS09520	2019049..2020266	-	1218	WP_161507136.1	J domain-containing protein	-
GWI30_RS09530	2020944..2022254	-	1311	WP_005329232.1	type II toxin-antitoxin system HipA family toxin	Toxin
GWI30_RS09535	2022254..2022508	-	255	WP_005329233.1	helix-turn-helix domain-containing protein	Antitoxin
GWI30_RS09540	2023010..2023258	+	249	WP_161507132.1	hypothetical protein	-
GWI30_RS09545	2023774..2024691	+	918	WP_005328758.1	IS5-like element ISAeme7 family transposase	-
GWI30_RS09550	2024750..2025001	-	252	WP_010674977.1	plasmid stabilization protein	-
GWI30_RS09555	2025080..2026462	-	1383	WP_201035452.1	Fic family protein	-
GWI30_RS09560	2026642..2027094	+	453	WP_021140686.1	DUF2384 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2023774..2024691	917

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 437 a.a.
• Molecular weight: 49096.31 Da
• Isoelectric Point: 7.1717

>T148588 WP_005329232.1 NZ_CP047962:c2022254-2020944 [Aeromonas media]
MASLTVYMNGYRVGTFTKATNGAHQFQYDESWVKLPGSRPISLSMPLRQQRYQGDEAYNFFDNLLPDNPEVRNRVVARYQ
ADSTQPFDLLSCIGQDSVGALQLVAQGRPVPDVKRIECKPLSDAELEQIMTSYQQGIPLGMVREEDDFRISIAGAQEKTA
LLYLDNRWCLPHAATPTTHIIKLPIGKIESHSYSIDLSQSVENEYLCTLIAKALGLPVPHCFIMQVGKVKALAVERFDRR
YATDRSWIMRLPQEDFCQVLNVPSARKYESHGGPGIADIMTTLLGSATPEQDRYLFMKSQVLFWLLAATDGHAKNFSVFI
EPEGRFRLTPFYDILSMYPAFGGKGLHPRDAKLAMGLTATKGKKYAIEQIFPRHFYQTAKAIGFEQVQMEKIISELTDNL
DEVISIVRQQLPDTFPTEIADSILGGLSSKAQRLKR

Nucleotide

• Length: 1311 bp

>T148588 NZ_CP063804:364360-364662 [Mycobacterium orygis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9271.66 Da
• Isoelectric Point: 9.1571

>AT148588 WP_005329233.1 NZ_CP047962:c2022508-2022254 [Aeromonas media]
MKVTSAKQLSACLKDARLSQKLSQGKVAGKVGIRQDTVSSFELNPESTKLETLFKILAALELSLDIRPRTDPAENATPGW
KEEW

Nucleotide

• Length: 255 bp

>AT148588 NZ_CP063804:364710-364931 [Mycobacterium orygis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Toxin

• AlphaFold DB: A0A2S4TP05