Detailed information of TA system TA147550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 1732868..1733397
• Replicon: chromosome
• Accession: NZ_CP047778
• Organism: Staphylococcus aureus strain UP_1572

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: K7ZRX6
• Locus tag: E3T13_RS08650
• Protein ID: WP_001103939.1
• Coordinates: 1732868..1733185 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: X5IA75
• Locus tag: E3T13_RS08655
• Protein ID: WP_001058492.1
• Coordinates: 1733188..1733397 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T13_RS08625	1728900..1729541	-	642	WP_160187057.1	pathogenicity island protein	-
E3T13_RS08630	1729543..1729806	-	264	WP_001804819.1	hypothetical protein	-
E3T13_RS08635	1729808..1730170	-	363	WP_001039165.1	hypothetical protein	-
E3T13_RS08640	1730449..1731918	-	1470	WP_053040304.1	virulence-associated E family protein	-
E3T13_RS08645	1731935..1732804	-	870	WP_160187058.1	primase alpha helix C-terminal domain-containing protein	-
E3T13_RS08650	1732868..1733185	-	318	WP_001103939.1	DUF1474 family protein	Toxin
E3T13_RS08655	1733188..1733397	-	210	WP_001058492.1	hypothetical protein	Antitoxin
E3T13_RS08660	1733390..1733536	-	147	WP_000784885.1	hypothetical protein	-
E3T13_RS08665	1733548..1733820	-	273	WP_053040301.1	helix-turn-helix domain-containing protein	-
E3T13_RS08670	1733821..1734033	-	213	WP_053040300.1	helix-turn-helix transcriptional regulator	-
E3T13_RS08675	1734183..1734917	+	735	WP_000142630.1	helix-turn-helix domain-containing protein	-
E3T13_RS08680	1735216..1736430	+	1215	WP_123090135.1	site-specific integrase	-
E3T13_RS08685	1736431..1737264	+	834	WP_180372434.1	type II toxin-antitoxin system PemK/MazF family toxin	-
E3T13_RS08690	1737496..1737738	-	243	WP_000897044.1	30S ribosomal protein S18	-
E3T13_RS08695	1737790..1738293	-	504	WP_000934799.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1718227..1739045	20818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12547.17 Da
• Isoelectric Point: 5.0161

>T147550 WP_001103939.1 NZ_CP047778:c1733185-1732868 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLHVYLKEFGELIQKF
HEIEKASSENFGEVSDDAQKLKITE

Nucleotide

• Length: 318 bp

>T147550 NZ_CP063046:c4335394-4335020 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8238.28 Da
• Isoelectric Point: 4.3718

>AT147550 WP_001058492.1 NZ_CP047778:c1733397-1733188 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVNGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT147550 NZ_CP063046:c4335851-4335483 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
CCAGCGATGCAGATGCGTACCCCCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCAATCAGCATACCGTCACGCTGTATGCGAAGGGGCTGGCCTGTGAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: K7ZRX6

Antitoxin

• AlphaFold DB: X5IA75