Detailed information of TA system TA147499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 310142..310738
• Replicon: chromosome
• Accession: NZ_CP047765
• Organism: Enterobacter hormaechei strain Eho-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A837F9W8
• Locus tag: GVI62_RS01495
• Protein ID: WP_023315213.1
• Coordinates: 310436..310738 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: GVI62_RS01490
• Protein ID: WP_023315212.1
• Coordinates: 310142..310429 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI62_RS01485	308514..310145	+	1632	WP_003860350.1	Na/Pi cotransporter family protein	-
GVI62_RS01490	310142..310429	-	288	WP_023315212.1	putative addiction module antidote protein	Antitoxin
GVI62_RS01495	310436..310738	-	303	WP_023315213.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GVI62_RS01500	310936..311808	+	873	WP_022646725.1	23S rRNA pseudouridine(2604) synthase RluF	-
GVI62_RS01505	311809..312081	-	273	WP_060580348.1	DUF3811 domain-containing protein	-
GVI62_RS01510	312132..313076	-	945	WP_022650218.1	ketopantoate/pantoate/pantothenate transporter PanS	-
GVI62_RS01515	313170..314519	-	1350	WP_003860344.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11427.21 Da
• Isoelectric Point: 10.1042

>T147499 WP_023315213.1 NZ_CP047765:c310738-310436 [Enterobacter hormaechei]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T147499 NZ_CP063008:c4791122-4790589 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10171.73 Da
• Isoelectric Point: 7.0086

>AT147499 WP_023315212.1 NZ_CP047765:c310429-310142 [Enterobacter hormaechei]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISLQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLRLTIKPSGD

Nucleotide

• Length: 288 bp

>AT147499 NZ_CP063008:c4791399-4791133 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A837F9W8