Detailed information of TA system TA147388

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 824960..825794
• Replicon: chromosome
• Accession: NZ_CP047710
• Organism: Escherichia coli strain Eco-15

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: GVI77_RS03955
• Protein ID: WP_001546109.1
• Coordinates: 824960..825337 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7I0L0C8
• Locus tag: GVI77_RS03960
• Protein ID: WP_001546108.1
• Coordinates: 825414..825794 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI77_RS03925	821354..821509	-	156	WP_000729639.1	hypothetical protein	-
GVI77_RS03930	821941..822875	-	935	Protein_772	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
GVI77_RS03935	822868..823263	-	396	WP_000208383.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
GVI77_RS03940	823332..824177	-	846	WP_001280441.1	DUF4942 domain-containing protein	-
GVI77_RS03945	824262..824459	-	198	WP_000839260.1	DUF957 domain-containing protein	-
GVI77_RS03950	824476..824963	-	488	Protein_776	hypothetical protein	-
GVI77_RS03955	824960..825337	-	378	WP_001546109.1	TA system toxin CbtA family protein	Toxin
GVI77_RS03960	825414..825794	-	381	WP_001546108.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GVI77_RS03965	825844..826488	-	645	WP_000086755.1	hypothetical protein	-
GVI77_RS03970	826507..826728	-	222	WP_000692345.1	DUF987 domain-containing protein	-
GVI77_RS03975	826797..827273	-	477	WP_001424026.1	RadC family protein	-
GVI77_RS03980	827289..827774	-	486	WP_000849588.1	antirestriction protein	-
GVI77_RS03985	827829..828647	-	819	WP_001234620.1	DUF945 domain-containing protein	-
GVI77_RS03990	828747..828980	-	234	WP_001119729.1	DUF905 family protein	-
GVI77_RS03995	829059..829514	-	456	WP_001545736.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13986.00 Da
• Isoelectric Point: 8.5190

>T147388 WP_001546109.1 NZ_CP047710:c825337-824960 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYKMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T147388 NZ_CP062940:c2167953-2167699 [Bifidobacterium longum subsp. longum]
ATGCTGCTCTGCTGGACCGAAGACGCCTGGGCCGACTACCTGTACTGGCAATCCCAGGACCGGAAGACCCTTAAACGCGT
CAACGCGCTCATACGGGATATGCAGCGTACCCCGTTCGAGGGCATCGGCAAGCCCGAGCCCCTCAAATGGGGCCTGTCCG
GCGCATGGTCGCGGCGCATCGACTCAGCCAACCGCATCATCTACACCGTCGCCGACGACAGGCTCTGCATCCTCTCCGCG
AAGGACCACTACTGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13997.78 Da
• Isoelectric Point: 5.0823

>AT147388 WP_001546108.1 NZ_CP047710:c825794-825414 [Escherichia coli]
MSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFNNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT147388 NZ_CP062940:c2168219-2167953 [Bifidobacterium longum subsp. longum]
ATGACACAGGCCGTGGCCTACAGCAATTTCAGGGCGAATCTCAAGACCTATATGCGCAGGGTCAACGAGGACGCCGACAC
CCTTCTCGTGACCAACGCGGACCCGGAGGACAACGTGGTCGTGATGAGCGCCGACGACTACGATTCGCTCATGGAGACGC
TGCGCGTCTACCAGAACCCGTATCTGAGCGACAAGGTGATGCGCGGCATGGCGCAGGTCCGGCAGGGCCAGACGATGGCC
CACGACCTTGTCGAAGCGGGCGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7I0L0C8