Detailed information of TA system TA147160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 87757..89380
• Replicon: plasmid pLD26-1-MCR1
• Accession: NZ_CP047666
• Organism: Escherichia coli strain LD26-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: H9TH67
• Locus tag: GUU86_RS23305
• Protein ID: WP_000088046.1
• Coordinates: 88055..89380 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: GUU86_RS23300
• Protein ID: WP_024136327.1
• Coordinates: 87757..88050 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU86_RS23275	83629..84870	+	1242	WP_001053338.1	VWA domain-containing protein	-
GUU86_RS23280	85433..85714	-	282	WP_000077926.1	hypothetical protein	-
GUU86_RS23285	85764..85955	-	192	WP_000398480.1	hypothetical protein	-
GUU86_RS23290	86047..86388	-	342	WP_001151575.1	hypothetical protein	-
GUU86_RS23295	86761..87153	+	393	WP_000341066.1	hypothetical protein	-
GUU86_RS23300	87757..88050	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
GUU86_RS23305	88055..89380	+	1326	WP_000088046.1	type II toxin-antitoxin system HipA family toxin	Toxin
GUU86_RS23310	89441..89647	+	207	WP_000134171.1	hypothetical protein	-
GUU86_RS23315	89749..90159	+	411	WP_000985911.1	hypothetical protein	-
GUU86_RS23320	90172..90987	+	816	WP_146881499.1	HNH endonuclease	-
GUU86_RS23325	91241..91666	+	426	WP_001043843.1	hypothetical protein	-
GUU86_RS23330	92215..92523	+	309	WP_001224687.1	hypothetical protein	-
GUU86_RS23335	92539..93396	+	858	WP_000784387.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mcr-1.1 / mph(A) / blaCTX-M-14 / fosA3 / aac(3)-IVa / sul2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / sul1	-	1..251000	251000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50249.20 Da
• Isoelectric Point: 6.1927

>T147160 WP_000088046.1 NZ_CP047666:88055-89380 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T147160 NZ_CP062871:c1049163-1048909 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT147160 WP_024136327.1 NZ_CP047666:87757-88050 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT147160 NZ_CP062871:c1049411-1049160 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A3T0VBU1

Antitoxin

• AlphaFold DB: A0A5Z6V2D1