Detailed information of TA system TA147150

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2342025..2342859
• Replicon: chromosome
• Accession: NZ_CP047665
• Organism: Escherichia coli strain LD26-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: GUU86_RS11265
• Protein ID: WP_021527014.1
• Coordinates: 2342482..2342859 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: GUU86_RS11260
• Protein ID: WP_032261140.1
• Coordinates: 2342025..2342393 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU86_RS11240	2339781..2340599	+	819	WP_032261137.1	DUF945 domain-containing protein	-
GUU86_RS11245	2340690..2341175	+	486	WP_021526886.1	antirestriction protein	-
GUU86_RS11250	2341191..2341667	+	477	WP_032261139.1	RadC family protein	-
GUU86_RS11255	2341730..2341951	+	222	WP_000692320.1	DUF987 domain-containing protein	-
GUU86_RS11260	2342025..2342393	+	369	WP_032261140.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
GUU86_RS11265	2342482..2342859	+	378	WP_021527014.1	type IV toxin-antitoxin system toxin CbtA	Toxin
GUU86_RS11270	2342856..2343005	+	150	Protein_2212	hypothetical protein	-
GUU86_RS11275	2343081..2343278	+	198	WP_089078746.1	DUF957 domain-containing protein	-
GUU86_RS11280	2343363..2343923	+	561	Protein_2214	DUF4942 domain-containing protein	-
GUU86_RS11285	2344290..2344826	-	537	WP_000942795.1	GspM family type II secretion system protein YghD	-
GUU86_RS11290	2344828..2346006	-	1179	WP_000094989.1	type II secretion system protein GspL	-
GUU86_RS11295	2346003..2346980	-	978	WP_000633239.1	type II secretion system minor pseudopilin GspK	-
GUU86_RS11300	2346977..2347582	-	606	WP_001255030.1	type II secretion system minor pseudopilin GspJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14010.13 Da
• Isoelectric Point: 9.1378

>T147150 WP_021527014.1 NZ_CP047665:2342482-2342859 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLGQHYGLTLDDTPFADERVIEQHIKAGISLCDAVNFLVEKYTLVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEVKR

Nucleotide

• Length: 378 bp

>T147150 NZ_CP062870:c74982-74776 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13682.60 Da
• Isoelectric Point: 6.6209

>AT147150 WP_032261140.1 NZ_CP047665:2342025-2342393 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRACIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT147150 NZ_CP062870:74969-75030 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT