Detailed information of TA system TA147147

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1797396..1798021
• Replicon: chromosome
• Accession: NZ_CP047665
• Organism: Escherichia coli strain LD26-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GUU86_RS08625
• Protein ID: WP_000911330.1
• Coordinates: 1797396..1797794 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: GUU86_RS08630
• Protein ID: WP_000450524.1
• Coordinates: 1797794..1798021 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU86_RS08605	1793274..1793474	+	201	WP_000383836.1	YpfN family protein	-
GUU86_RS08610	1793584..1794282	-	699	WP_000679823.1	esterase	-
GUU86_RS08615	1794356..1796371	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
GUU86_RS08620	1796386..1797249	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
GUU86_RS08625	1797396..1797794	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GUU86_RS08630	1797794..1798021	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
GUU86_RS08635	1798175..1798888	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
GUU86_RS08640	1799101..1800135	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
GUU86_RS08645	1800152..1801030	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
GUU86_RS08650	1801176..1801748	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
GUU86_RS08655	1801748..1802218	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T147147 WP_000911330.1 NZ_CP047665:c1797794-1797396 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T147147 NZ_CP062869:15459-15740 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT147147 WP_000450524.1 NZ_CP047665:c1798021-1797794 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT147147 NZ_CP062869:15193-15462 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33