Detailed information of TA system TA147123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 3820181..3820779
• Replicon: chromosome
• Accession: NZ_CP047662
• Organism: Escherichia coli strain LD93-1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: GUU84_RS18135
• Protein ID: WP_053270478.1
• Coordinates: 3820181..3820558 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: GUU84_RS18140
• Protein ID: WP_001603498.1
• Coordinates: 3820558..3820779 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU84_RS18115	3817322..3818029	+	708	WP_000234514.1	DUF554 domain-containing protein	-
GUU84_RS18125	3818408..3819670	+	1263	WP_089634553.1	integrase arm-type DNA-binding domain-containing protein	-
GUU84_RS18130	3819856..3820071	+	216	Protein_3535	transposase	-
GUU84_RS18135	3820181..3820558	-	378	WP_053270478.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GUU84_RS18140	3820558..3820779	-	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GUU84_RS18145	3820964..3821974	-	1011	WP_160188256.1	fimbrial protein	-
GUU84_RS18150	3821985..3824471	-	2487	WP_160188257.1	fimbria/pilus outer membrane usher protein	-
GUU84_RS18155	3824489..3825169	-	681	WP_053270475.1	fimbrial assembly chaperone	-
GUU84_RS18160	3825225..3825773	-	549	WP_076486551.1	fimbrial protein YehD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3819856..3829944	10088
	-	flank	IS/Tn	-	-	3819856..3820137	281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13419.20 Da
• Isoelectric Point: 6.7260

>T147123 WP_053270478.1 NZ_CP047662:c3820558-3820181 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMSDSGRAEAIIGRVQARVAYEEITGLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T147123 NZ_CP062867:c69808-69410 [Escherichia coli O39:H21]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT147123 WP_001603498.1 NZ_CP047662:c3820779-3820558 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT147123 NZ_CP062867:c70035-69808 [Escherichia coli O39:H21]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: L4J1C0