Detailed information of TA system TA146888

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 109330..109584
• Replicon: plasmid pTrh52_1
• Accession: NZ_CP047601
• Organism: Escherichia coli O84:H7 strain Trh52

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: GUK18_RS23385
• Protein ID: WP_001351576.1
• Coordinates: 109330..109536 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	109523..109584 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUK18_RS23360	104952..105488	-	537	WP_050576192.1	type 1 fimbrial protein	-
GUK18_RS23365	106772..106927	-	156	WP_032145292.1	hypothetical protein	-
GUK18_RS23370	107628..108485	-	858	WP_021572483.1	incFII family plasmid replication initiator RepA	-
GUK18_RS23375	108478..108552	-	75	WP_032144212.1	RepA leader peptide Tap	-
GUK18_RS23380	108798..109046	-	249	WP_000083837.1	replication regulatory protein RepA	-
GUK18_RS23385	109330..109536	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	109523..109584	+	62	NuclAT_1	-	Antitoxin
-	109523..109584	+	62	NuclAT_1	-	Antitoxin
-	109523..109584	+	62	NuclAT_1	-	Antitoxin
-	109523..109584	+	62	NuclAT_1	-	Antitoxin
GUK18_RS23390	109841..109915	-	75	Protein_92	endonuclease	-
GUK18_RS23395	110161..110373	-	213	WP_001299730.1	ANR family transcriptional regulator	-
GUK18_RS23400	110509..111069	-	561	WP_001587438.1	fertility inhibition protein FinO	-
GUK18_RS23405	111124..111870	-	747	WP_000205759.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	papH / papC / papD / hlyB / iroB / iroC / iroD / iroE / iroN / faeJ / faeI / faeH / faeF / faeF / faeE / faeD / faeC	1..158248	158248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T146888 WP_001351576.1 NZ_CP047601:c109536-109330 [Escherichia coli O84:H7]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T146888 NZ_CP062820:137346-137603 [Escherichia coli]
GTGTTTAGTCACCGCATTGTGGTCTCGGCCATTACTTACTCTGAAATGCGCTTCGGTGCCTCCGGTCCGAAGGCCTCCCC
GCGTCACGTGCAACTGGTTGATAAATTCTGCCCGTGCCTCGATGCCATCCTGTCTTGGGACCGCGCCGCGGTGGACGGCA
CCACGGATATTAAAGTGGCACTGCGCCAGGCCGGGACGCCGATCGGCCAGAACGATACGGCGATTGCCGGGCCTGAAGCT
GGACGTATAGGAAATTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT146888 NZ_CP047601:109523-109584 [Escherichia coli O84:H7]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin