Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3887844..3888394 Replicon chromosome
Accession NZ_CP047600
Organism Escherichia coli O84:H7 strain Trh52

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0K4H9R5
Locus tag GUK18_RS18645 Protein ID WP_024223381.1
Coordinates 3888080..3888394 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag GUK18_RS18640 Protein ID WP_000125566.1
Coordinates 3887844..3888077 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUK18_RS18615 3882917..3883204 + 288 WP_000203741.1 ferredoxin-like protein FixX -
GUK18_RS18620 3883263..3884594 + 1332 WP_001183207.1 MFS transporter -
GUK18_RS18625 3884702..3885232 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
GUK18_RS18630 3885225..3887087 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
GUK18_RS18635 3887279..3887758 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
GUK18_RS18640 3887844..3888077 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
GUK18_RS18645 3888080..3888394 + 315 WP_024223381.1 CcdB family protein Toxin
GUK18_RS18650 3888391..3889239 - 849 WP_024223380.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
GUK18_RS18655 3889246..3889623 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
GUK18_RS18660 3889626..3890447 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
GUK18_RS18665 3890444..3891433 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
GUK18_RS18670 3891433..3892719 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T146884 WP_024223381.1 NZ_CP047600:3888080-3888394 [Escherichia coli O84:H7]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMVDGI

Download         Length: 315 bp

>T146884 NZ_CP062820:c75653-75372 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT146884 WP_000125566.1 NZ_CP047600:3887844-3888077 [Escherichia coli O84:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT146884 NZ_CP062820:c75919-75650 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGGCAGACCGTTGAACAGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCCGCTACTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4H9R5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References