Detailed information of TA system TA146881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3218473..3219235
• Replicon: chromosome
• Accession: NZ_CP047600
• Organism: Escherichia coli O84:H7 strain Trh52

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0K5JE43
• Locus tag: GUK18_RS15570
• Protein ID: WP_028125934.1
• Coordinates: 3218945..3219235 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GUK18_RS15565
• Protein ID: WP_203164317.1
• Coordinates: 3218473..3218928 (-)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUK18_RS15535	3213763..3214659	+	897	WP_000621626.1	sulfofructose kinase	-
GUK18_RS15540	3214693..3215478	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
GUK18_RS15545	3215577..3216176	+	600	WP_001298594.1	glucose-1-phosphatase	-
GUK18_RS15550	3216170..3217042	+	873	WP_000920762.1	virulence factor BrkB family protein	-
GUK18_RS15555	3217039..3217476	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GUK18_RS15560	3217521..3218462	+	942	WP_024223284.1	fatty acid biosynthesis protein FabY	-
GUK18_RS15565	3218473..3218928	-	456	WP_203164317.1	helix-turn-helix domain-containing protein	Antitoxin
GUK18_RS15570	3218945..3219235	-	291	WP_028125934.1	type II toxin-antitoxin system HigB family toxin	Toxin
GUK18_RS15575	3219634..3219852	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
GUK18_RS15580	3220074..3221003	-	930	WP_203164318.1	formate dehydrogenase accessory protein FdhE	-
GUK18_RS15585	3221000..3221635	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GUK18_RS15590	3221632..3222534	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (95-146)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11608.45 Da
• Isoelectric Point: 8.8235

>T146881 WP_028125934.1 NZ_CP047600:c3219235-3218945 [Escherichia coli O84:H7]
MHVISHKPFNEAIKRFPPYEGALIDLLNLLEKTEFSDPGEMKRYIPSLDNFKYRDKWWVINIAGNHLRLIAYIDFRLQKC
FIKHTVIYNEYDRFTT

Nucleotide

• Length: 291 bp

>T146881 NZ_CP062817:2543-264 [Escherichia coli]

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 17148.57 Da
• Isoelectric Point: 6.6011

>AT146881 WP_203164317.1 NZ_CP047600:c3218928-3218473 [Escherichia coli O84:H7]
MRTHHTPAEDSANFGMLVKTFTHAVKDIPLCGPKADQEEYHNALKLIEFLVDRDDLENPLFELLCVRIREYENNAPEFQH
FNQRLASTPCGVSLLRILMDQHGLKAADLAQELGSKSNVNNILNGRRALTVRHIKALSERFNLPAEAFIDK

Nucleotide

• Length: 456 bp

>AT146881 NZ_CP062817:261-641 [Escherichia coli]
ATGAAAAAAGATAAAAAATATCAAATAGAAGCAATAAAAAATAAAGATAAAACTTTATTTATTGTCTATGCTACTGATAT
TTATAGCCCGAGCGAATTTTTCTCAAAAATCGAATCCGACTTGAAGAAAAAGAAAAGCAAGGGTGATGTTTTTTTTGATT
TAATAATTCCTAACGGTGGAAAAAAAGATCGTTACGTCTATACGTCATTTAATGGCGAGAAGTTTTCAAGTTACACATTA
AACAAAGTTACGAAAACTGATGAATATAATGATTTATCTGAGCTCTCGGCTTCGTTCTTTAAAAAGAACTTTGATAAGAT
CAACGTAAACCTTCTATCCAAAGCCACATCATTTGCTTTGAAAAAAGGCATTCCAATATAA

Structures

Toxin

• AlphaFold DB: A0A0K5JE43