Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2367499..2368298 Replicon chromosome
Accession NZ_CP047600
Organism Escherichia coli O84:H7 strain Trh52

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag GUK18_RS11420 Protein ID WP_000347251.1
Coordinates 2367834..2368298 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0K4DCM8
Locus tag GUK18_RS11415 Protein ID WP_016233578.1
Coordinates 2367499..2367834 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUK18_RS11400 2363284..2364054 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
GUK18_RS11405 2364070..2365404 - 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
GUK18_RS11410 2365779..2367350 + 1572 WP_203164480.1 galactarate dehydratase -
GUK18_RS11415 2367499..2367834 + 336 WP_016233578.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GUK18_RS11420 2367834..2368298 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GUK18_RS11425 2368353..2369162 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
GUK18_RS11430 2369411..2370691 + 1281 WP_000681941.1 tagatose-bisphosphate aldolase subunit KbaZ -
GUK18_RS11435 2370714..2371187 + 474 WP_001298322.1 PTS N-acetylgalactosamine transporter subunit IIB -
GUK18_RS11440 2371198..2371977 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
GUK18_RS11445 2371967..2372845 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
GUK18_RS11450 2372863..2373297 + 435 WP_000948837.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T146879 WP_000347251.1 NZ_CP047600:2367834-2368298 [Escherichia coli O84:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T146879 NZ_CP062815:c4267968-4267795 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12335.94 Da        Isoelectric Point: 4.5392

>AT146879 WP_016233578.1 NZ_CP047600:2367499-2367834 [Escherichia coli O84:H7]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLIAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT146879 NZ_CP062815:c4268292-4267996 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0K4DCM8

References